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A novel technique for oviduct occlusion to generate live births from cryopreserved rabbit oocytes after in vivo fertilisation

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A novel technique for oviduct occlusion to generate live births from cryopreserved rabbit oocytes after in vivo fertilisation

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dc.contributor.author Jiménez Trigos, María Estrella es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.date.accessioned 2016-07-11T12:41:04Z
dc.date.available 2016-07-11T12:41:04Z
dc.date.issued 2014-08
dc.identifier.issn 0378-4320
dc.identifier.uri http://hdl.handle.net/10251/67411
dc.description.abstract [EN] Intraoviductal transfer technique in combination with in vivo fertilisation has arisen as an effective technique to assess live births after transfer of slow-frozen oocytes in the rabbit. Nevertheless, the great disadvantage of this method is the accumulation of tubal fluid in a large number of females after clamping the oviducts. In this study, we develop an alternative method to minimise damage to the oviduct and increase the birth rate. The aims of this study were (1) to evaluate the ability of cyanoacrylate tissue adhesive to occlude the oviduct for female sterilisation; (2) to evaluate the effect of oviduct occlusion immediately after transferring fresh oocytes on in vivo fertilisation; and (3) to assess this technique to generate live births from fresh and slow-frozen oocytes. In all the experiments, recipients were artificially inseminated 9 h prior to occluding the oviducts. In the first experiment, the left oviduct was blocked with cyanoacrylate tissue adhesive, while the right one was used as a control. Six days later, oviducts and uterine horns were flushed to assess embryo recovery rates. While the embryo recovery rate was 79.2% in the intact oviduct, no embryos were recovered in the blocked one. In the second experiment, fresh oocytes were transferred into both oviducts, which were immediately occluded. Six days later, the in vivo fertilisation success rate was 33.7%. Finally, in the last experiment, slow-frozen oocytes were transferred and the rate of live births was 13.2 ± 4.5%. The study shows that when using this method the generation of live births from slow-frozen oocytes increases significantly. In addition, our results suggest that in vivo environment could help improve the results of oocyte cryopreservation es_ES
dc.description.sponsorship This work was supported by the Spanish Research Project AGL2011-30170-C02-01 (CICYT) and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009/125). Estrella Jimenez was supported by a research grant from the Education Ministry of the Valencian Regional Government (programme VALi+d. ACIF/2010/262). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier Masson es_ES
dc.relation.ispartof Animal Reproduction Science es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit es_ES
dc.subject Blocked oviduct es_ES
dc.subject Cyanoacrylate tissue adhesive es_ES
dc.subject Laparoscopy Intraoviductal transfer es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title A novel technique for oviduct occlusion to generate live births from cryopreserved rabbit oocytes after in vivo fertilisation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.anireprosci.2014.06.007
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2011-30170-C02-02/ES/ESTUDIO DEL SISTEMA INMUNE DE DIFERENTES LINEAS GENETICAS CUNICOLAS Y SU RESPUESTA FRENTE A DESAFIOS PRODUCTIVOS E INFECCIOSOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2009%2F125/ES/Efecto de la crioconservación de embriones sobre el desarrollo y el re-establecimiento de poblaciones/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2010%2F262/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Jiménez Trigos, ME.; Vicente Antón, JS.; Marco Jiménez, F. (2014). A novel technique for oviduct occlusion to generate live births from cryopreserved rabbit oocytes after in vivo fertilisation. Animal Reproduction Science. 148(3-4):197-204. https://doi.org/10.1016/j.anireprosci.2014.06.007 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.anireprosci.2014.06.007 es_ES
dc.description.upvformatpinicio 197 es_ES
dc.description.upvformatpfin 204 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 148 es_ES
dc.description.issue 3-4 es_ES
dc.relation.senia 268625 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES


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