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Platelet-rich plasma favors proliferation of canine adipose-derived mesenchymal stem cells in methacrylate-endcapped caprolactone porous scaffold niches

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Platelet-rich plasma favors proliferation of canine adipose-derived mesenchymal stem cells in methacrylate-endcapped caprolactone porous scaffold niches

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dc.contributor.author Rodriguez-Jimenez, Francisco Javier es_ES
dc.contributor.author Valdes-Sanchez, Teresa es_ES
dc.contributor.author Carrillo, Jose M. es_ES
dc.contributor.author Rubio, Mónica es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.contributor.author García Cruz, Dunia Mercedes es_ES
dc.contributor.author Garcia, Montserrat es_ES
dc.contributor.author Cugat, Ramón es_ES
dc.contributor.author Moreno-Manzano, Victoria es_ES
dc.date.accessioned 2016-07-14T12:01:10Z
dc.date.available 2016-07-14T12:01:10Z
dc.date.issued 2012
dc.identifier.issn 2079-4983
dc.identifier.uri http://hdl.handle.net/10251/67601
dc.description.abstract [EN] Osteoarticular pathologies very often require an implementation therapy to favor regeneration processes of bone, cartilage and/or tendons. Clinical approaches performed on osteoarticular complications in dogs constitute an ideal model for human clinical translational applications. The adipose-derived mesenchymal stem cells (ASCs) have already been used to accelerate and facilitate the regenerative process. ASCs can be maintained in vitro and they can be differentiated to osteocytes or chondrocytes offering a good tool for cell replacement therapies in human and veterinary medicine. Although ACSs can be easily obtained from adipose tissue, the amplification process is usually performed by a time consuming process of successive passages. In this work, we use canine ASCs obtained by using a Bioreactor device under GMP cell culture conditions that produces a minimum of 30 million cells within 2 weeks. This method provides a rapid and aseptic method for production of sufficient stem cells with potential further use in clinical applications. We show that plasma rich in growth factors (PRGF) treatment positively contributes to viability and proliferation of canine ASCs into caprolactone 2-(methacryloyloxy) ethyl ester (CLMA) scaffolds. This biomaterial does not need additional modifications for cASCs attachment and proliferation. Here we propose a framework based on a combination of approaches that may contribute to increase the therapeutical capability of stem cells by the use of PRGF and compatible biomaterials for bone and connective tissue regeneration. es_ES
dc.description.sponsorship This research was funded by the Fundación García-Cugat and the Government of Spain.
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Journal of Functional Biomaterials es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject adipose tissue es_ES
dc.subject mesenchymal stem cells es_ES
dc.subject PRGF es_ES
dc.subject caprolactone es_ES
dc.subject scaffold es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Platelet-rich plasma favors proliferation of canine adipose-derived mesenchymal stem cells in methacrylate-endcapped caprolactone porous scaffold niches es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/jfb3030556
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Rodriguez-Jimenez, FJ.; Valdes-Sanchez, T.; Carrillo, JM.; Rubio, M.; Monleón Pradas, M.; García Cruz, DM.; Garcia, M.... (2012). Platelet-rich plasma favors proliferation of canine adipose-derived mesenchymal stem cells in methacrylate-endcapped caprolactone porous scaffold niches. Journal of Functional Biomaterials. 3(3):556-568. doi:10.3390/jfb3030556 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3390/jfb3030556 es_ES
dc.description.upvformatpinicio 556 es_ES
dc.description.upvformatpfin 568 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 3 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 241947 es_ES
dc.identifier.pmid 24955632 en_EN
dc.identifier.pmcid PMC4030998 en_EN
dc.contributor.funder Fundación García Cugat
dc.contributor.funder Gobierno de España


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