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Transcription profiling of different Arabidopsis thaliana ecotypes in response to Tobacco etch potyvirus infection

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Transcription profiling of different Arabidopsis thaliana ecotypes in response to Tobacco etch potyvirus infection

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dc.contributor.author Hillung, Julia es_ES
dc.contributor.author Cuevas Torrijos, Jose Manuel es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2016-07-18T12:23:53Z
dc.date.available 2016-07-18T12:23:53Z
dc.date.issued 2012-06
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10251/67743
dc.description.abstract The use of high-throughput transcript profiling techniques has opened the possibility of identifying, in a single experiment, multiple host mRNAs whose levels of accumulation are altered in response to virus infection. Several studies have used this approach to analyze the response of Arabidopsis thaliana to the infection by different RNA and DNA viruses. However, the possible differences in response of genetically heterogeneous ecotypes of the plant to the same virus have never been addressed before. Here we have used a strain ofTobacco etch potyvirus (TEV) experimentally adapted to A. thaliana ecotype Ler-0 and a set of seven plant ecotypes to tackle this question. Each ecotype was inoculated with the same amount of the virus and the outcome of infection characterized phenotypically (i.e., virus infectivity, accumulation, and symptoms development). Using commercial microarrays containing probes for more than 43,000 A. thaliana transcripts, we explored the effect of viral infection on the plant transcriptome. In general, we found that ecotypes differ in the way they perceive and respond to the virus. Some ecotypes developed strong symptoms and accumulated large amounts of viral genomes, while others only developed mild symptoms and accumulated less virus. At the transcriptomic level, ecotypes could be classified into two groups according to the particular genes whose expression was altered upon infection. Moreover, a functional enrichment analyses showed that the two groups differed in the nature of the altered biological processes. For the group constituted by ecotypes developing milder symptoms and allowing for lower virus accumulation, genes involved in abiotic stresses and in the construction of new tissues tend to be up-regulated. For those ecotypes in which infection was more severe and productive, defense genes tend to be up-regulated, deviating the necessary resources from building new tissues. es_ES
dc.description.sponsorship We thank Francisca de la Iglesia, Javier Forment, and Maria A. Martinez-Godoy for excellent technical assistance; Miguel A. Perez-Amador and Francisco Vera for guidance in the use of Agilent microarrays; and Guillermo Rodrigo for help with microarray analyses. This work was supported by grant BFU2009-06993 from the Spanish Ministerio de Ciencia e Innovacion (MICINN) to Santiago F. Elena. Julia Hillung was supported by a fellowship from MICINN. Jose M. Cuevas was contracted under the CSIC JAE-doc program. en_EN
dc.language Inglés es_ES
dc.publisher Frontiers Media es_ES
dc.relation Spanish Ministerio de Ciencia e Innovación BFU2009-06993 es_ES
dc.relation CSIC JAE-doc program es_ES
dc.relation.ispartof Frontiers in Microbiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Emerging viruses es_ES
dc.subject Experimental evolution es_ES
dc.subject Host-virus interaction es_ES
dc.subject Systems biology es_ES
dc.subject Virus evolution es_ES
dc.title Transcription profiling of different Arabidopsis thaliana ecotypes in response to Tobacco etch potyvirus infection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fmicb.2012.00229
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hillung, J.; Cuevas Torrijos, JM.; Elena Fito, SF. (2012). Transcription profiling of different Arabidopsis thaliana ecotypes in response to Tobacco etch potyvirus infection. Frontiers in Microbiology. 3(229):1-9. doi:10.3389/fmicb.2012.00229 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3389/fmicb.2012.00229 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 3 es_ES
dc.description.issue 229 es_ES
dc.relation.senia 232213 es_ES


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