- -

A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L

Show simple item record

Files in this item

dc.contributor.author Seguí-Simarro, Jose M. es_ES
dc.contributor.author Corral Martínez, Patricia es_ES
dc.contributor.author Corredor, E. es_ES
dc.contributor.author Raska, I. es_ES
dc.contributor.author Testillano, P. S. es_ES
dc.contributor.author Risueno, M.C. es_ES
dc.date.accessioned 2016-07-19T06:46:38Z
dc.date.available 2016-07-19T06:46:38Z
dc.date.issued 2011-05-15
dc.identifier.issn 0176-1617
dc.identifier.uri http://hdl.handle.net/10251/67776
dc.description.abstract After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. The architecture of the well-organized nuclear functional domains changes in response to DNA replication, RNA transcription, processing and transport dynamics. A number of subnuclear structures present in the interchromatin region (IR, the nuclear domain between chromosome territories) have been shown as involved, either directly or indirectly, in transcriptional regulation. These structures include the interchromatin granule clusters (IGCs), perichromatin fibrils (PFs), Cajal bodies (CBs) and perichromatin granules (PGs). In this work, we present a cytochemical, immunocytochemical, quantitative and morphometric analysis at the light, confocal and electron microscopy levels to characterize the changes in the functional architecture of the nuclear interchromatin domain during two developmental programs followed by the microspore: differentiation to mature pollen grains (transcriptionally inactive), and microspore embryogenesis involving proliferation in the first stages (highly engaged in transcription). Our results revealed characteristic changes in size, shape and distribution of the different interchromatin structures as a consequence of the reprogramming of the microspore, allowing us to relate the remodeling of the interchromatin domain to the variations in transcriptional activities during proliferation and differentiation events, and suggesting that RNA-associated structures could be a regulatory mechanism in the process. In addition, we document the presence of two structurally different types of CBs, and of IGC and CB-associated regions, similar to those present in animal cells, and not yet described in plants. © 2010 Elsevier GmbH. es_ES
dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Education and Science AGL2006-06678 to J.M.S.S., AGL2008-04255 to M.C.R. BFU2008-00203 to P.S.T. and Czech grants MSM0021620806, LC535 and AV0Z50110509 to I.R. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cajal bodies es_ES
dc.subject Differentiation es_ES
dc.subject Maturation es_ES
dc.subject Microspore embryogenesis es_ES
dc.subject Nucleus es_ES
dc.subject Pollen es_ES
dc.subject Proliferation es_ES
dc.subject Rapeseed es_ES
dc.subject Article es_ES
dc.subject Bacterial spore es_ES
dc.subject Biological model es_ES
dc.subject Cell differentiation es_ES
dc.subject Cell nucleus es_ES
dc.subject Cell proliferation es_ES
dc.subject Chromatin es_ES
dc.subject Coiled body es_ES
dc.subject Confocal microscopy es_ES
dc.subject Frozen section es_ES
dc.subject Genetics es_ES
dc.subject Haploidy es_ES
dc.subject Immunoelectron microscopy es_ES
dc.subject Immunohistochemistry es_ES
dc.subject Metabolism es_ES
dc.subject Physiological stress es_ES
dc.subject Physiology es_ES
dc.subject Prenatal development es_ES
dc.subject RNA processing es_ES
dc.subject Ultrastructure es_ES
dc.subject Brassica napus es_ES
dc.subject Coiled Bodies es_ES
dc.subject Frozen Sections es_ES
dc.subject Microscopy, Confocal es_ES
dc.subject Microscopy, Immunoelectron es_ES
dc.subject Models, Biological es_ES
dc.subject RNA Processing, Post-Transcriptional es_ES
dc.subject Spores es_ES
dc.subject Stress, Physiological es_ES
dc.subject Animalia es_ES
dc.subject.classification GENETICA es_ES
dc.title A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jplph.2010.10.014
dc.relation.projectID info:eu-repo/grantAgreement/MEC//AGL2006-06678/ES/OBTENCION DE LINEAS DOBLE HAPLOIDES EN SOLANACEAS DE ELEVADO INTERES AGRONOMICO: ANALISIS DE AGENTES INDUCTORES Y MECANISMOS CELULARES IMPLICADOS EN LA INDUCCION EMBRIOGENICA EN TOMATE Y BERENJENA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04255/ES/EMBRIOGENESIS DE POLEN Y DOBLE-HAPLOIDES PARA MEJORA DE ESPECIES DE INTERES AGRONOMICO: IDENTIFICACION DE FACTORES ESTIMULANTES E INHIBIDORES DE EMBRIOGENESIS Y ANALISIS TRANSCRIPTOMICO Y PROTEOMICO./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2008-00203/ES/REPROGRAMACION DEL POLEN A EMBRIOGENESIS INDUCIDA POR ESTRES EN PLANTAS DE INTERES ECONOMICO: FISIOLOGIA,TRANSCRIPTOMICA Y EPIGENETICA DE LA RESPUESTA A ESTRES, DESDIFERENCIACION Y COMPETENCIA EMBRIOG/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MSMT//MSM0021620806/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MSMT//LC535/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MSMT//AV0Z50110509/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Seguí-Simarro, JM.; Corral Martínez, P.; Corredor, E.; Raska, I.; Testillano, PS.; Risueno, M. (2011). A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L. Journal of Plant Physiology. 168(8):746-757. https://doi.org/10.1016/j.jplph.2010.10.014 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jplph.2010.10.014 es_ES
dc.description.upvformatpinicio 746 es_ES
dc.description.upvformatpfin 757 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 168 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 198305 es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministry of Education, Youth and Sport of the Czech Republic


This item appears in the following Collection(s)

Show simple item record