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Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

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Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

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dc.contributor.author López Gresa, Mª Pilar es_ES
dc.contributor.author Lisón Párraga, María Purificación es_ES
dc.contributor.author Kim, Hye Kyong es_ES
dc.contributor.author Choi, Young Hae es_ES
dc.contributor.author Verpoorte, R es_ES
dc.contributor.author Rodrigo Bravo, Ismael es_ES
dc.contributor.author Conejero Tomás, Vicente es_ES
dc.contributor.author Belles Albert, José Mª es_ES
dc.date.accessioned 2016-07-20T10:36:24Z
dc.date.available 2016-07-20T10:36:24Z
dc.date.issued 2012-11-01
dc.identifier.issn 0176-1617
dc.identifier.uri http://hdl.handle.net/10251/67895
dc.description.abstract [EN] 1H nuclear magnetic resonance (NMR)-based metabolomics has been applied to study the compatible interaction between tomato plants and Tomato Mosaic Virus (ToMV). A detailed time course of metabolic fingerprinting of ToMV-inoculated and non-inoculated systemically infected tomato leaves has provided a fundamental understanding of the metabolic state of the plant not only in response to ToMV infection, but also under various physiological conditions. By this analytical platform a total of 32 metabolites including amino/organic acids, sugars, phenylpropanoids, flavonoids and other miscellaneous compounds were detected. Using multivariate data analysis, we have identified a subset of metabolites induced during the plant defence response and metabolites whose accumulation was dependent on the developmental stage, the position of the leaf on the stem, and the harvesting time. Specifically, a general time-dependent decrease in organic acids, amino acids (excluding asparagine), phenylpropanoids and rutin was observed in individual leaves. In addition, metabolite alterations were also found to correlate with the developmental stage of the leaf: high levels of organic acids, some amino acids, phenylpropanoids, and flavonoids were found in lower leaves while elevated amounts of sugars were present in the upper ones. Moreover, a marked variation in the content of some metabolites was also observed to be associated to the asymptomatic ToMV infection both in inoculated and systemically infected leaves. While flavonoids accumulated in virus-inoculated leaves, increased levels of phenylpropanoids were observed in non-inoculated leaves where ToMV actively replicates. Finally, diurnal changes in the metabolite content were also observed: an increase of amino acids and organic acids (except glutamic acid) were observed in the samples collected in the morning, whereas sugars and secondary metabolite levels increased in the tomato leaves harvested in the evening es_ES
dc.description.sponsorship The authors are grateful to Cristina Torres Vidal for technical support and Dr. Lynne Yenush for critical reading of the manuscript and exciting intellectual discussions. This work was supported by Grant BFU 2009-11958 from Secretaria de Estado de Investigacion from Spanish Ministry of Science and Innovation. M.P.L.G held a postdoctoral fellowship JAEDoc 08 00402 from the Consejo Superior de Investigaciones Cientificas (Spain) and was recipient of a postdoctoral fellowship (BEST/2010/111) from Generalitat Valencia (Spain). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Solanum lycopersicum es_ES
dc.subject Tomato Mosaic Virus (ToMV) es_ES
dc.subject Plant pathogen interaction es_ES
dc.subject Systemic acquired resistance (SAR) es_ES
dc.subject NMR-based metabolomics es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jplph.2012.05.021
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2009-11958/ES/Señalizacion Y Respuesta Defensiva De Las Plantas Frente A Patogenos/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CSIC//JAEDoc 08 00402/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//BEST%2F2010%2F111/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation López Gresa, MP.; Lisón Párraga, MP.; Kim, HK.; Choi, YH.; Verpoorte, R.; Rodrigo Bravo, I.; Conejero Tomás, V.... (2012). Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum. Journal of Plant Physiology. 169(16):1586-1596. https://doi.org/10.1016/j.jplph.2012.05.021 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jplph.2012.05.021 es_ES
dc.description.upvformatpinicio 1586 es_ES
dc.description.upvformatpfin 1596 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 169 es_ES
dc.description.issue 16 es_ES
dc.relation.senia 231123 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES


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