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Function of glutathione peroxidases in legume root nodules

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Function of glutathione peroxidases in legume root nodules

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dc.contributor.author Matamoros, Manuel A. es_ES
dc.contributor.author SAIZ ANDRES, ANA es_ES
dc.contributor.author Peñuelas, Maria es_ES
dc.contributor.author Bustos-Sanmamed, Pilar es_ES
dc.contributor.author Mulet Salort, José Miguel es_ES
dc.contributor.author Barja, Maria V. es_ES
dc.contributor.author Rouhier, Nicolas es_ES
dc.contributor.author Moore, Marten es_ES
dc.contributor.author James, Euan K. es_ES
dc.contributor.author Dietz, Karl-Josef es_ES
dc.contributor.author Becana, Manuel es_ES
dc.date.accessioned 2016-07-20T11:42:25Z
dc.date.available 2016-07-20T11:42:25Z
dc.date.issued 2015-05
dc.identifier.issn 0022-0957
dc.identifier.uri http://hdl.handle.net/10251/67899
dc.description © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. es_ES
dc.description.abstract [EN] Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function. es_ES
dc.description.sponsorship AS and PBS were the recipients of predoctoral (Formacion de Personal Investigador) and postdoctoral (Marie Curie) contracts, respectively. We thank Martin Crespi for help with in situ RNA hybridization and Simon Avery for sharing the yeast mutant and for helpful advice. This work was supported by Ministerio de Economia y Competitividad-Fondo Europeo de Desarrollo Regional (AGL2011-24524 and AGL2014-53717-R). The UMR1136 is supported by a grant overseen by the French National Research Agency (ANR) as part of the 'Investissements d'Avenir' programme (ANR-11-LABX-0002-01, Lab of Excellence ARBRE). MM and KJD acknowledge support within SPP1710. The proteomic analysis was performed in the CSIC/UAB Proteomics Facility of IIBB-CSIC that belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001. en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//AGL2011-24524/ES/SEÑALIZACION POR ESPECIES REACTIVAS DE OXIGENO%2FNITROGENO Y ANTIOXIDANTES EN LA SIMBIOSIS FIJADORA DE NITROGENO RHIZOBIUM-LEGUMINOSA/ es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//AGL2014-53717-R/ES/FIJACION DE NITROGENO POR LA SIMBIOSIS RIZOBIO-LEGUMINOSA: HEMOGLOBINAS Y MODIFICACIONES OXIDATIVAS DE LAS PROTEINAS DURANTE EL DESARROLLO Y SENESCENCIA DE LOS NODULOS/ es_ES
dc.relation French National Research Agency (ANR) as part of the 'Investissements d'Avenir' programme ANR-11-LABX-0002-01 es_ES
dc.relation SPP1710 es_ES
dc.relation PT13/0001 es_ES
dc.relation FPI es_ES
dc.relation Marie Curie es_ES
dc.relation.ispartof Journal of Experimental Botany es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Antioxidants es_ES
dc.subject Glutathione peroxidases es_ES
dc.subject Legume nodules es_ES
dc.subject Lotus japonicus es_ES
dc.subject Nitric oxide es_ES
dc.subject Reactive oxygen species es_ES
dc.subject S-nitrosylation es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Function of glutathione peroxidases in legume root nodules es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/jxb/erv066
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Matamoros, MA.; Saiz Andres, A.; Peñuelas, M.; Bustos-Sanmamed, P.; Mulet Salort, JM.; Barja, MV.; Rouhier, N.... (2015). Function of glutathione peroxidases in legume root nodules. Journal of Experimental Botany. 66(10):2979-2990. https://doi.org/10.1093/jxb/erv066 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1093/jxb/erv066 es_ES
dc.description.upvformatpinicio 2979 es_ES
dc.description.upvformatpfin 2990 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 66 es_ES
dc.description.issue 10 es_ES
dc.relation.senia 290024 es_ES
dc.identifier.eissn 1460-2431
dc.identifier.pmid 25740929 en_EN
dc.identifier.pmcid PMC4423513 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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