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Development and characterization of genomic simple sequence repeat markers in eggplant and their application to the study of diversity and relationships in a collection of different cultivar types and origins

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Development and characterization of genomic simple sequence repeat markers in eggplant and their application to the study of diversity and relationships in a collection of different cultivar types and origins

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dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Manzur Poblete, Juan Pablo Andrés es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.date.accessioned 2016-07-27T06:56:33Z
dc.date.available 2016-07-27T06:56:33Z
dc.date.issued 2012
dc.identifier.issn 1380-3743
dc.identifier.uri http://hdl.handle.net/10251/68253
dc.description.abstract The eggplant (Solanum melongena L.) genome is the least investigated among the economically most important solanaceous crops. Extensive use of molecular markers will improve eggplant germplasm enhancement and breeding. Microsatellites, or simple sequence repeats, have proved to be very useful for eggplant germplasm management and breeding, but there is limited availability of these polymorphic, codominant, and highly repeatable markers in eggplant. We developed a genomic DNA library enriched with AG/CT, which allowed the identification of 55 new genomic microsatellites. Variation parameters of microsatellite loci analyzed showed high average values. The potential of these markers for fingerprinting was assessed in a collection of 24 accessions, of which 22 correspond to S. melongena from different types (landraces, heirlooms, modern F1 hybrids, and obsolete cultivars) and origins, and two to each of the cultivated relatives S. aethiopicum and S. macrocarpon. The multivariate (cluster and PCoA) analyses clearly differentiated four main clusters: (a) two outgroups formed by S. aethiopicum and S. macrocarpon accessions, (b) S. melongena accessions derived mostly from the Mediterranean basin, Central Europe, Africa, and America (‘occidental’ eggplants), and (c) S. melongena accessions derived mostly from Eastern and Southeastern Asia (‘oriental’ eggplants). However, no apparent association pattern was found for accessions of the different types. Observed heterozygosity (Ho) values were low, although hybrid cultivars had higher values (Ho = 0.12) than non-hybrid materials (Ho = 0.02). The new set of eggplant microsatellite markers has proved highly informative and useful for studying the diversity, relationships, and genetic characteristics of an eggplant collection. These markers will be useful for germplasm management and breeding in eggplant. es_ES
dc.description.sponsorship This work was funded by the Ministerio de Ciencia y Tecnologia (AGL2009-07257 and RF-2008-00008-00-00), Generalitat Valenciana (ACOMP/2011/032) and Universitat Politecnica de Valencia (PAID-05-10-2318). en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Ministerio de Ciencia y Tecnologı´a (AGL2009-07257 and RF-2008- 00008-00-00) es_ES
dc.relation Generalitat Valenciana (ACOMP/2011/032) es_ES
dc.relation Universitat Politècnica de València (PAID-05-10-2318). es_ES
dc.relation.ispartof Molecular Breeding es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Eggplant es_ES
dc.subject Microsatellites es_ES
dc.subject Simple sequence repeat es_ES
dc.subject Solanum melongena es_ES
dc.subject Genetic diversity es_ES
dc.subject.classification GENETICA es_ES
dc.title Development and characterization of genomic simple sequence repeat markers in eggplant and their application to the study of diversity and relationships in a collection of different cultivar types and origins es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11032-011-9650-2
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Vilanova Navarro, S.; Manzur Poblete, JPA.; Prohens Tomás, J. (2012). Development and characterization of genomic simple sequence repeat markers in eggplant and their application to the study of diversity and relationships in a collection of different cultivar types and origins. Molecular Breeding. 30:647-660. doi:10.1007/s11032-011-9650-2 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s11032-011-9650-2 es_ES
dc.description.upvformatpinicio 647 es_ES
dc.description.upvformatpfin 660 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 30 es_ES
dc.relation.senia 236739 es_ES
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