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Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

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Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

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dc.contributor.author Viudes De Castro, María Pilar es_ES
dc.contributor.author Lavara García, Raquel es_ES
dc.contributor.author Safaa, Hosam Mohamed Ali Ahmed es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.contributor.author MEHAISEN, GAMAL MOHAMED KAMEL OSMAN es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2016-07-29T07:58:32Z
dc.date.available 2016-07-29T07:58:32Z
dc.date.issued 2014-05
dc.identifier.issn 1751-7311
dc.identifier.uri http://hdl.handle.net/10251/68414
dc.description.abstract [EN] This study was conducted to elucidate the effect of different freezing extenders on two lines selected for hyperprolificacy and longevity (H and LP, respectively). In extender A, dimethyl sulphoxide (Me2SO) and sucrose were used as cryoprotectants. In extenders B and C, the sucrose was replaced by 20% egg yolk, and in extender C the Me2SO was substituted by acetamide. Semen was packaged in 0.25 ml plastic straws and cooled at 5°C for 45 min, and then was frozen in liquid nitrogen vapour for 10 min before being plunged into the liquid nitrogen. Thawing was carried out by immersing the straws in a water bath at 50°C for 10 s. Frozen-thawed semen characteristics and reproductive parameters were affected by freezing. Extender C showed significantly lower post-thawing quality traits than any of the three extenders. Acrosome integrity was significantly improved when Me2SO was used as cryoprotectant. Sucrose replacement by 20% egg yolk had no effect on acrosome integrity but provided significantly lower sperm motility and viability. Freezing extender affected fertility rate, total born, number of implantation sites and gestational losses, obtaining better results when extender A was used. The acrosomal integrity after frozen-thawed process showed a significant correlation with fertility at 12th day and also at birth, indicating that an increase in acrosomal integrity leads to an increase in both fertilities (12th day and at birth). A positive correlation between motility of semen and implantation sites was found. The post-thawing quality traits of semen were not affected by the genetic line, although LP line showed higher total born and lower foetal and gestational losses. The findings of this study suggest that freezing extender composition has a significant effect on the success of rabbit sperm for preservation, and when Me2SO was used as permeable cryoprotectant sucrose provided better protection compared with egg yolk and improved reproductive traits, and, on the other hand, the male genotypes used in the present study had no effect on frozen-thawed sperm parameters but negatively affected some of the reproductive parameters es_ES
dc.description.sponsorship This research was supported partly by the RTA2010-00117-00-00 from INIA, the European FEDER Funds and 125-2009 Prometeo Project (Generalitat Valenciana). The authors thank Neil Macowan Language Services for revising the English version of the manuscript.
dc.language Inglés es_ES
dc.publisher Cambridge University Press (CUP): STM Journals es_ES
dc.relation.ispartof Animal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Rabbit es_ES
dc.subject Spermatozoa es_ES
dc.subject Freezing extender es_ES
dc.subject Genotype es_ES
dc.subject Fertility es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1017/S1751731114000135
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2010-00117-00-00/ES/Incorporación de análogos sintéticos de GnRH a los diluyentes de inseminación para simplificar la técnica de inseminación artificial en cunicultura y mejora de los protocolos y diluyentes de crioconservación de semen./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO09%2F2009%2F125/ES/Efecto de la crio conservación de embriones sobre el desarrollo y el restablecimiento de poblaciones/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Viudes De Castro, MP.; Lavara García, R.; Safaa, HMAA.; Marco Jiménez, F.; Mehaisen, GMKO.; Vicente Antón, JS. (2014). Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits. Animal. 8(5):765-770. https://doi.org/10.1017/S1751731114000135 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1017/S1751731114000135 es_ES
dc.description.upvformatpinicio 765 es_ES
dc.description.upvformatpfin 770 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 257419 es_ES
dc.identifier.eissn 1751-732X
dc.identifier.pmid 24534625
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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