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Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis

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Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis

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dc.contributor.author Vilchez Olivencia, Maria Carmen es_ES
dc.contributor.author Santangeli, Stefania es_ES
dc.contributor.author Maradonna, Francesca es_ES
dc.contributor.author Gioacchini, Giorgia es_ES
dc.contributor.author Verdenelli, Cristina es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Tveiten, Helge es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Carnevali, Oliana es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2016-09-01T07:07:04Z
dc.date.available 2016-09-01T07:07:04Z
dc.date.issued 2015-11
dc.identifier.issn 0093-691X
dc.identifier.uri http://hdl.handle.net/10251/68473
dc.description.abstract [EN] Positive effects of probiotics on fish reproduction have been reported in several species. In the present study, 40 male European eels were weekly treated with recombinant hCG for 9 weeks and with three different concentrations (10(3), 10(5), and 10(6) CFU/mL) of probiotic Lactobacillus rhamnosus IMC 501 (Sinbyotec, Italy). The probiotics were daily added to the water from the sixth week of the hCG treatment. Males from the treated and control groups were sacrificed after 1, 2, and 3 weeks of probiotic treatment (seventh ninth weeks of hCG treatment); at this point, sperm and testis samples were also collected. Sperm volume was estimated, and motility was analyzed by computer-assisted sperm analysis software. Alternations in transcription of specific genes involved in reproductive process such as activin, androgen receptors alpha and beta (ar alpha and ar beta), progesterone receptor 1 (pr1), bone morphogenetic protein 15 (bmp15), and FSH receptor (fshr) were analyzed in the testis. After 2 weeks of probiotic treatment, sperm production and sperm motility parameters (percentage of motile cells and percentage of straight-swimming spermatozoa) were increased in the European eel treated with 105 CFU/mL compared to controls or to the other probiotic doses. These changes were associated with increases in messenger RNA expression of activin, ar alpha, ar beta, pr1, and fshr. Conversely, after 3 weeks, activin and pr1 expression decreased. No significant changes were observed on bmp15 expression throughout the duration of the treatment with 10(5) CFU/mL dose. The lowest and highest probiotic dose (10(3) and 10(6) CFU/mL, respectively) inhibited the transcription of all genes along all the experiment, except for ar alpha and ar beta after 1 week of probiotic treatment when compared to controls. The changes observed by transcriptomic analysis and the sperm parameters suggest that a treatment with L rhamnosus at 10(5) CFU/mL for 2 weeks could improve spermatogenesis process in Anguilla anguilla. (C) 2015 Elsevier Inc. All rights reserved. es_ES
dc.description.sponsorship This study was funded by the European Community’s 7th FP (grant agreement no. 245257, PRO-EEL) and COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE) Victor Gallego and M. Carmen Vilchez have predoctoral grants from MINISTERIO DE ECONOMIA Y COMPETITIVIDAD (BES-2009-020310) and UNIVERSIDAD POLITECNICA DE VALENCIA PAID Program (2011-S2-02-6521), respectively. Fondo Ateneo 2012 to Oliana Carnevali. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation EU/COST/FA1205: AQUAGAMETE es_ES
dc.relation MINECO/BES-2009-020310 es_ES
dc.relation UPV/PAID/2011-S2-02-6521 es_ES
dc.relation.ispartof Theriogenology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Anguilla anguilla es_ES
dc.subject Probiotic es_ES
dc.subject Lactobacillus rhamnosus es_ES
dc.subject Messenger RNA level es_ES
dc.subject Computer-assisted sperm analysis system es_ES
dc.subject Spermatogenesis es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.theriogenology.2015.07.011
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/245257 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Vilchez Olivencia, MC.; Santangeli, S.; Maradonna, F.; Gioacchini, G.; Verdenelli, C.; Gallego Albiach, V.; Peñaranda, D.... (2015). Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis. Theriogenology. 84(8):1321-1331. https://doi.org/10.1016/j.theriogenology.2015.07.011 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.theriogenology.2015.07.011 es_ES
dc.description.upvformatpinicio 1321 es_ES
dc.description.upvformatpfin 1331 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 84 es_ES
dc.description.issue 8 es_ES
dc.relation.senia 292408 es_ES
dc.identifier.eissn 1879-3231
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Universitat Politècnica de València


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