- -

Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCZ1) increase through induced spermatogenesis in European eel

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCZ1) increase through induced spermatogenesis in European eel

Show simple item record

Files in this item

dc.contributor.author Morini, Marina Ange Marie es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Vilchez Olivencia, Maria Carmen es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Nourizadeh-Lillabadi, Rasoul es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.contributor.author Weltzien, Finn-Arne es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.date.accessioned 2016-09-01T12:34:54Z
dc.date.available 2016-09-01T12:34:54Z
dc.date.issued 2015-09
dc.identifier.issn 1095-6433
dc.identifier.uri http://hdl.handle.net/10251/68535
dc.description.abstract [EN] Activation at fertilization of the vertebrate egg is triggered by Ca2+ waves. Recent studies suggest the phospholipase C zeta (PLC zeta), a sperm-specific protein, triggers egg activation by an 1P3-mediated Ca2+ release and allow Ca2+ waves at fertilization. In the present study we cloned, characterized, and phylogenetically positioned the European eel PLC zeta (PLC zeta 1). It is 1521bp long, with 10 exons encoding an open reading frame of 506 amino acids. The amino acid sequence contains an EF-hand domain, X and Y catalytic domains, and a carboxy-terminal C2 domain, all typical of other PLC zeta orthologous. The tissue distribution was studied, and the gene expression was determined in testis during induced sexual maturation at three different thermal regimes. Also, brain and pituitary expression was studied through sex maturation at constant temperature. plc zeta was expressed in brain of male and female, in testis but not in ovaries. By first time in vertebrates, it is reported plc zeta 1 expression in the pituitary gland. Testis plc zeta 1 expression increased through spermatogenesis under all the thermal regimes, but being significantly elevated at lower temperatures. It was very low when testis contained only spermatogonia or spermatocytes, while maximum expression was found during spermiogenesis. These results support the hypothesis for an eel sperm-specific PLC zeta inducing egg activation, similarly to mammals and some teleosts, but different from some other teleost species, which express this protein in ovaries, but not in testes. (C) 2015 Elsevier Inc. All rights reserved. es_ES
dc.description.sponsorship Funded by the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vílchez has a predoctoral grant from UPV PAID Programme (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolía, GRISOLIA/2012/006), Victor Gallego has a postdoctoral grant (UPV; PAID-10-14), and David S. Peñaranda was a contract cofinanced by MICINN and UPV (PTA2011-4948-I). Grants to attend meetings from COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE).
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//AGL2010-16009/ES/MECANISMOS FISIOLOGICOS IMPLICADOS EN LA ESPERMIACION Y EN LA ADQUISICION DE MOTILIDAD ESPERMATICA EN LA ANGUILA EUROPEA (ANGUILLA ANGUILLA)/ es_ES
dc.relation UPV/PAID/2011-S2-02-6521 es_ES
dc.relation GV/GRISOLIA/2012/006 es_ES
dc.relation UPV/PAID-10-14 es_ES
dc.relation UPV/PTA2011-4948-I es_ES
dc.relation EU/COSTAction/FA1205 es_ES
dc.relation.ispartof Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Teleost es_ES
dc.subject Reproduction es_ES
dc.subject Fertilization es_ES
dc.subject Spermatozoa es_ES
dc.subject Anguilla anguilla es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCZ1) increase through induced spermatogenesis in European eel es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cpba.2015.05.028
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Morini, MAM.; Peñaranda, D.; Vilchez Olivencia, MC.; Gallego Albiach, V.; Nourizadeh-Lillabadi, R.; Asturiano Nemesio, JF.; Weltzien, F.... (2015). Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCZ1) increase through induced spermatogenesis in European eel. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 187:168-176. https://doi.org/10.1016/j.cpba.2015.05.028 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.cbpa.2015.05.028 es_ES
dc.description.upvformatpinicio 168 es_ES
dc.description.upvformatpfin 176 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 187 es_ES
dc.relation.senia 292407 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Universitat Politècnica de València
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder European Commission


This item appears in the following Collection(s)

Show simple item record