Gramaje, D.; Mostert, L.; Armengol Fortí, J. (2011). Characterization of Cadophora luteo-olivacea and C. melinii isolates obtained from grapevines and environmental samples from grapevine nurseries in Spain. Phytopathologia Mediterranea. 50(Supplement):112-126. https://doi.org/10.14601/Phytopathol_Mediterr-8723
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/68574
Title:
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Characterization of Cadophora luteo-olivacea and C. melinii isolates obtained from grapevines and environmental samples from grapevine nurseries in Spain
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Author:
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Gramaje, David
Mostert, Lizel
Armengol Fortí, Josep
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
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Issued date:
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Abstract:
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[EN] Fifty-eight Cadophora luteo-olivacea and three C. melinii isolates were recovered from grapevines showing black vascular streaking and decline symptoms characteristic of Petri disease, and from different stages of the ...[+]
[EN] Fifty-eight Cadophora luteo-olivacea and three C. melinii isolates were recovered from grapevines showing black vascular streaking and decline symptoms characteristic of Petri disease, and from different stages of the grapevine nursery process in Spain. The isolates were studied by means of phenotypical characterization, DNA analysis and pathogenicity tests. The morphological characters studied included conidiophore, phialide and conidial morphology. Colony characters and pigment production on MEA, PDA and OA were also examined. Phenotypical data were subjected to cluster analysis, which clearly separated C. luteo-olivacea isolates into four groups. Mating tests were performed on all possible combinations for each Cadophora species but no sexual fruiting bodies were produced. Partial sequences of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (BT) and the elongation factor 1¿ (EF) were analysed, but no genetic variation occurred within the C. luteo-olivacea isolates or within the C. melinii isolates in any of the regions studied. Pathogenicity tests were conducted on 1-year-old grapevine cuttings of four different rootstocks using four C. luteo-olivacea isolates and one isolate of C. melinii. All Cadophora isolates except the C. melinii isolate caused significantly longer lesions in the xylem of grapevine rootstocks than in the controls.
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Subjects:
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Cadophora species
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Grapevine nurseries
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Vitis vinifera
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Young vineyards
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Cadophora
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Cadophora luteo-olivacea
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Cadophora melinii
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Vitaceae
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Vitis
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Copyrigths:
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Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
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Source:
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Phytopathologia Mediterranea. (issn:
0031-9465
)
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DOI:
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10.14601/Phytopathol_Mediterr-8723
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Publisher:
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Firenze University Press
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Publisher version:
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http://www.fupress.net/index.php/pm/article/view/8723
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Project ID:
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info:eu-repo/grantAgreement/MICINN//RTA2010-00009-C03-03/ES/RTA2010-00009-C03-03/
info:eu-repo/grantAgreement/MEC//RTA2007-00023-C04-03/ES/Biología y desarrollo de métodos de control de hongos de madera de la vid en planta joven./
info:eu-repo/grantAgreement/MEC//AGL2006-11884-C04-01/ES/BIOLOGIA Y DESARROLLO DE METODOS DE CONTROL DE HONGOS DE MADERA DE LA VID EN PLANTA JOVEN./
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Thanks:
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This research was financially supported by the Projects AGL2006-11884-C04-01 (Ministerio de Educación y Ciencia, Spain), RTA2007-00023-C04-03 and RTA2010-00009-C03-03 (Programa Nacional de Recursos y Tecnologías Agrarias, ...[+]
This research was financially supported by the Projects AGL2006-11884-C04-01 (Ministerio de Educación y Ciencia, Spain), RTA2007-00023-C04-03 and RTA2010-00009-C03-03 (Programa Nacional de Recursos y Tecnologías Agrarias, Ministerio de Educación y Ciencia, Spain), and the European Regional Development Fund (ERDF). We thank V. Garrigues for technical assistance.
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Type:
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Artículo
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