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The single-subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling

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The single-subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling

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dc.contributor.author Bueso Ródenas, Eduardo es_ES
dc.contributor.author Rodriguez, Lesia es_ES
dc.contributor.author Lorenzo Orts, Laura es_ES
dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Sayas Montañana, Enric Miquel es_ES
dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Ibañez, Carla es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.date.accessioned 2016-09-05T07:55:08Z
dc.date.available 2016-09-05T07:55:08Z
dc.date.issued 2014-12
dc.identifier.issn 0960-7412
dc.identifier.uri http://hdl.handle.net/10251/68682
dc.description.abstract [EN] Membrane-delimited events play a crucial role for ABA signaling and PYR/PYL/RCAR ABA receptors, clade A PP2Cs and SnRK2/CPK kinases modulate the activity of different plasma membrane components involved in ABA action. Therefore, the turnover of PYR/PYL/RCARs in the proximity of plasma membrane might be a step that affects receptor function and downstream signaling. In this study we describe a single-subunit RING-type E3 ubiquitin ligase RSL1 that interacts with the PYL4 and PYR1 ABA receptors at the plasma membrane. Overexpression of RSL1 reduces ABA sensitivity and rsl1 RNAi lines that impair expression of several members of the RSL1/RFA gene family show enhanced sensitivity to ABA. RSL1 bears a C-terminal transmembrane domain that targets the E3 ligase to plasma membrane. Accordingly, bimolecular fluorescent complementation (BiFC) studies showed the RSL1–PYL4 and RSL1–PYR1 interaction is localized to plasma membrane. RSL1 promoted PYL4 and PYR1 degradation in vivo and mediated in vitro ubiquitylation of the receptors. Taken together, these results suggest ubiquitylation of ABA receptors at plasma membrane is a process that might affect their function via effect on their half-life, protein interactions or trafficking. es_ES
dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas (grants BIO2011-23446 to P.L.R.; BFU2011-22526 to R.S.; fellowship to L.R.; fellowship UPV to L.L-O; JAE-DOC contract to M.G.G.). We acknowledge Professor Joerg Kudla (University of Munster, Germany) for kindly providing plasma membrane marker OFP-TM23. Technical assistance of Maria A. Fernandez is greatly acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject ABA receptor es_ES
dc.subject Protein turnover es_ES
dc.subject RING E3 ubiquitin ligase es_ES
dc.subject RFA gene family es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title The single-subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/tpj.12708
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-23446/ES/SEÑALIZACION DE ABA MEDIADA POR LOS RECEPTORES PYR%2FPYL Y SU CONEXION CON LOS MECANISMOS DE RESISTENCIA A SEQUIA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-22526/ES/NUEVOS MECANISMOS DE TRANSMISION DE SEÑALES DURANTE EL METABOLISMO DE GLUCOSA Y LA ACIDIFICACION INTRACELULAR: AMPLIANDO LAS FUNCIONES DE LA PROTEINA FOSFATASA 1 Y LA PROTEINA/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Bueso Ródenas, E.; Rodriguez, L.; Lorenzo Orts, L.; González Guzmán, M.; Sayas Montañana, EM.; Muñoz Bertomeu, J.; Ibañez, C.... (2014). The single-subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling. Plant Journal. 80(6):1057-1071. https://doi.org/10.1111/tpj.12708 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/tpj.12708 es_ES
dc.description.upvformatpinicio 1057 es_ES
dc.description.upvformatpfin 1071 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 80 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 287868 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
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