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ARABIDOPSIS THALIANA HOMEOBOX25 uncovers a role for gibberellins in seed longevity

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ARABIDOPSIS THALIANA HOMEOBOX25 uncovers a role for gibberellins in seed longevity

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dc.contributor.author Bueso Ródenas, Eduardo es_ES
dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Campos, Francisco es_ES
dc.contributor.author Brunaud, Veronique es_ES
dc.contributor.author Martinez, Liliam es_ES
dc.contributor.author Sayas Montañana, Enric Miquel es_ES
dc.contributor.author Ballester Fuentes, Patricia es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.date.accessioned 2016-09-05T08:04:59Z
dc.date.available 2016-09-05T08:04:59Z
dc.date.issued 2014-02
dc.identifier.issn 0032-0889
dc.identifier.uri http://hdl.handle.net/10251/68684
dc.description.abstract [EN] Seed longevity is crucial for agriculture and plant genetic diversity, but it is limited by cellular damage during storage. Seeds are protected against aging by cellular defenses and by structures such as the seed coat. We have screened an activation-tagging mutant collection of Arabidopsis (Arabidopsis thaliana) and selected four dominant mutants with improved seed longevity (isl1-1D to isl4-1D) under both natural and accelerated aging conditions. In the isl1-1D mutant, characterized in this work, overexpression of the transcription factor ARABIDOPSIS THALIANA HOMEOBOX25 (ATHB25; At5g65410) increases the expression of GIBBERELLIC ACID3-OXIDASE2, encoding a gibberellin (GA) biosynthetic enzyme, and the levels of GA(1) and GA(4) are higher (3.2- and 1.4-fold, respectively) in the mutant than in the wild type. The morphological and seed longevity phenotypes of the athb25-1D mutant were recapitulated in transgenic plants with moderate (4- to 6-fold) overexpression of ATHB25. Simultaneous knockdown of ATHB25, ATHB22, and ATHB31 expression decreases seed longevity, as does loss of ATHB25 and ATHB22 function in a double mutant line. Seeds from wild-type plants treated with GA and from a quintuple DELLA mutant (with constitutive GA signaling) are more tolerant to aging, providing additional evidence for a role of GA in seed longevity. A correlation was observed in several genotypes between seed longevity and mucilage formation at the seed surface, suggesting that GA may act by reinforcing the seed coat. This mechanism was supported by the observation of a maternal effect in reciprocal crosses between the wild type and the athb25-1D mutant. es_ES
dc.description.sponsorship This work was supported by the Spanish Ministerio de Economia y Competividad (grant no. EUI2009-03985 and Juan de la Cierva contract to J.M-B.) as well as by the Consejo Nacional de Ciencia y Tecnologia and Direccion General de Asuntos del Personal Academico, Universidad Nacional Autonoma de Mexico (sabbatical fellowship to F.C.). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//EUI2009-03985/ES/ADVANCING YIELD STABILITY AND RESOURCE EFFICIENCY OF CROP PLANTS/ es_ES
dc.relation Spanish Ministerio de Economia y Competividad (Juan de la Cierva contract) es_ES
dc.relation Consejo Nacional de Ciencia y Tecnologia es_ES
dc.relation Direccion General de Asuntos del Personal Academico, Universidad Nacional Autonoma de Mexico es_ES
dc.relation.ispartof Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject CONTROLLED DETERIORATION es_ES
dc.subject EMBRYO DEVELOPMENT es_ES
dc.subject PLANT DEVELOPMENT es_ES
dc.subject ARABIDOPSIS es_ES
dc.subject GERMINATION es_ES
dc.subject STRESS es_ES
dc.subject PROTEIN es_ES
dc.subject GENE es_ES
dc.subject BIOSYNTHESIS es_ES
dc.subject EXPRESSION es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title ARABIDOPSIS THALIANA HOMEOBOX25 uncovers a role for gibberellins in seed longevity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1104/pp.113.232223
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Bueso Ródenas, E.; Muñoz Bertomeu, J.; Campos, F.; Brunaud, V.; Martinez, L.; Sayas Montañana, EM.; Ballester Fuentes, P.... (2014). ARABIDOPSIS THALIANA HOMEOBOX25 uncovers a role for gibberellins in seed longevity. Plant Physiology. 164(2):999-1010. https://doi.org/10.1104/pp.113.232223 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1104/pp.113.232223 es_ES
dc.description.upvformatpinicio 999 es_ES
dc.description.upvformatpfin 1010 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 164 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 270131 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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