- -

Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cells in Arabidopsis

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cells in Arabidopsis

Show simple item record

Files in this item

dc.contributor.author Anoman, Armand D es_ES
dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Rosa-Tellez, Sara es_ES
dc.contributor.author Flores-Tornero, María es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.contributor.author Bueso Ródenas, Eduardo es_ES
dc.contributor.author Fernie, Alisdair R es_ES
dc.contributor.author Segura, Juan es_ES
dc.contributor.author Ros Palau, Roc es_ES
dc.date.accessioned 2016-09-05T08:11:43Z
dc.date.available 2016-09-05T08:11:43Z
dc.date.issued 2015-11
dc.identifier.issn 0032-0889
dc.identifier.uri http://hdl.handle.net/10251/68686
dc.description.abstract This study functionally characterizes the Arabidopsis (Arabidopsis thaliana) plastidial glycolytic isoforms of glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in photosynthetic and heterotrophic cells. We expressed the enzyme in gapcp double mutants (gapcp1gapcp2) under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 had no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affected Arabidopsis development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Our results indicate that GAPCp1 is not functionally important in photosynthetic cells but plays a fundamental role in roots and in heterotrophic cells of the AP. Specifically, GAPCp activity may be required in root meristems and the root cap for normal primary root growth. Transcriptomic and metabolomic analyses indicate that the lack of GAPCp activity affects nitrogen and carbon metabolism as well as mineral nutrition and that glycerate and glutamine are the main metabolites responding to GAPCp activity. Thus, GAPCp could be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development. es_ES
dc.description.sponsorship This work was supported by the Spanish Government and the European Union (Fondo Europeo de Desarrollo Regional grant no. BFU2012-31519 to J.M.-B., Formacion del Personal Investigador fellowship to S.R.-T., and Agencia Espanola de Cooperacion Internacional fellowship to A.D.A.), by the Valencian Regional Government (PROMETEO grant no. II/2014/052), and by the University of Valencia (Atraccio de Talent fellowship to M.F.-T.). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation Spanish Government es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//BFU2012-31519/ES/CARACTERIZACION FUNCIONAL DE ENZIMAS CLAVE DEL METABOLISMO PLASTIDIAL EN ARABIDOPSIS Y MAIZ COMO ESTRATEGIA PARA MEJORAR LA CALIDAD NUTRICIONAL DE LAS PLANTAS/ es_ES
dc.relation Valencian Regional Government (PROMETEO) II/2014/052 es_ES
dc.relation University of Valencia (Atraccio de Talent fellowship) es_ES
dc.relation.ispartof Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cells in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1104/pp.15.00696
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Anoman, AD.; Muñoz Bertomeu, J.; Rosa-Tellez, S.; Flores-Tornero, M.; Serrano Salom, R.; Bueso Ródenas, E.; Fernie, AR.... (2015). Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cells in Arabidopsis. Plant Physiology. 169(3):1619-1637. https://doi.org/10.1104/pp.15.00696 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1104/pp.15.00696 es_ES
dc.description.upvformatpinicio 1619 es_ES
dc.description.upvformatpfin 1637 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 169 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 308438 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


This item appears in the following Collection(s)

Show simple item record