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Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy

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Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy

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dc.contributor.author Sarrion-Perdigones, Alejandro es_ES
dc.contributor.author Palací, Jorge es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2016-09-05T08:22:53Z
dc.date.available 2016-09-05T08:22:53Z
dc.date.issued 2014
dc.identifier.issn 1064-3745
dc.identifier.uri http://hdl.handle.net/10251/68688
dc.description.abstract GoldenBraid (GB) is an iterative and standardized DNA assembling system specially designed for Multigene Engineering in Plant Synthetic Biology. GB is based on restriction–ligation reactions using type IIS restriction enzymes. GB comprises a collection of standard DNA pieces named “GB parts” and a set of destination plasmids (pDGBs) that incorporate the multipartite assembly of standardized DNA parts. GB reactions are extremely efficient: two transcriptional units (TUs) can be assembled from several basic GBparts in one T-DNA less than 24 h. Moreover, larger assemblies comprising 4–5 TUs are routinely built in less than 2 working weeks. Here we provide a detailed view of the GB methodology. As a practical example, a Bimolecular Fluorescence Complementation construct comprising four TUs in a 12 kb DNA fragment is presented. es_ES
dc.language Inglés es_ES
dc.publisher Humana Press es_ES
dc.relation.ispartof Methods in Molecular Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Synthetic biology es_ES
dc.subject DNA assembly es_ES
dc.subject Type IIS enzymes es_ES
dc.subject Plant biotechnology es_ES
dc.subject Multigene constructs es_ES
dc.subject Multigene engineering es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/978-1-62703-764-8_10
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Sarrion-Perdigones, A.; Palací, J.; Granell Richart, A.; Orzáez Calatayud, DV. (2014). Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy. Methods in Molecular Biology. 1116:133-151. doi:10.1007/978-1-62703-764-8_10 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/978-1-62703-764-8_10 es_ES
dc.description.upvformatpinicio 133 es_ES
dc.description.upvformatpfin 151 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 1116 es_ES
dc.relation.senia 256762 es_ES
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