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A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance

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A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance

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dc.contributor.author Martínez Macías, Félix es_ES
dc.contributor.author Arif, Anjuman es_ES
dc.contributor.author González Nebauer, Sergio es_ES
dc.contributor.author Bueso Ródenas, Eduardo es_ES
dc.contributor.author Ali, Rashid es_ES
dc.contributor.author Montesinos De Lago, Consuelo es_ES
dc.contributor.author Brunaud, Veronique es_ES
dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Serrano Salom, Ramón
dc.date.accessioned 2016-09-05T08:24:31Z
dc.date.available 2016-09-05T08:24:31Z
dc.date.issued 2015
dc.identifier.issn 0032-0935
dc.identifier.uri http://hdl.handle.net/10251/68689
dc.description.abstract [EN] A fungal gene encoding a transcription factor is expressed from its own promoter in Arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. Horizontal gene transfer from unrelated organisms has occurred in the course of plant evolution, suggesting that some foreign genes may be useful to plants. The CtHSR1 gene, previously isolated from the halophytic yeast Candida tropicalis, encodes a heat-shock transcription factor-related protein. CtHSR1, with expression driven by its own promoter or by the Arabidopsis UBQ10 promoter, was introduced into the model plant Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation and the resulting transgenic plants were more tolerant to drought than controls. Fusions of the CtHSR1 promoter with beta-glucuronidase reporter gene indicated that this fungal promoter drives expression to phloem tissues. A chimera of CtHSR1 and green fluorescence protein is localized at the cell nucleus. The physiological mechanism of drought tolerance in transgenic plants is based on reduced transpiration (which correlates with decreased opening of stomata and increased levels of jasmonic acid) and increased osmotic potential (which correlates with increased proline accumulation). Transcriptomic analysis indicates that the CtHSR1 transgenic plants overexpressed a hundred of genes, including many relevant to stress defense such as LOX4 (involved in jasmonic acid synthesis) and P5CS1 (involved in proline biosynthesis). The promoters of the induced genes were enriched in upstream activating sequences for water stress induction. These results demonstrate that genes from unrelated organisms can have functional expression in plants from its own promoter and expand the possibilities of useful transgenes for plant biotechnology. es_ES
dc.description.sponsorship We acknowledge support by Grants BFU2011-22526 of the Spanish MICINN (Madrid, Spain) and PROMETEO II 2014-041 of Generalitat Valenciana (Valencia, Spain). J. M.-B. was supported by a Juan de la Cierva contract of the Spanish MICINN. A. A. was supported by a short-term EMBO fellowship to visit the laboratory of R. Serrano. We thank Dr. Jose Maria Belles (IBMCP, Valencia, Spain) for assistance in the determination of sugars, Dr. Isabel Lopez-Diaz and Dr. Esther Carrera for the hormone analysis carried out at the Plant Hormone Quantification Service of IBMCP and Prof. Jorg Kudla (Westfalische Wilhelms-Universitat, Munster, Germany) for the pGPTVII.Hyg.P<INF>UBQ10</INF>::MCS plasmid. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Planta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arabidopsis es_ES
dc.subject Candida tropicalis es_ES
dc.subject Horizontal gene transfer es_ES
dc.subject Jasmonic acid es_ES
dc.subject Proline es_ES
dc.subject Water relations es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.subject.classification FISIOLOGIA VEGETAL es_ES
dc.title A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00425-015-2285-5
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-22526/ES/NUEVOS MECANISMOS DE TRANSMISION DE SEÑALES DURANTE EL METABOLISMO DE GLUCOSA Y LA ACIDIFICACION INTRACELULAR: AMPLIANDO LAS FUNCIONES DE LA PROTEINA FOSFATASA 1 Y LA PROTEINA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F041/ES/La homeostasis de cationes monovalentes (H+, K+ y Na+) y el crecimiento y muerte celular/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.description.bibliographicCitation Martínez Macías, F.; Arif, A.; González Nebauer, S.; Bueso Ródenas, E.; Ali, R.; Montesinos De Lago, C.; Brunaud, V.... (2015). A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance. Planta. 242(1):39-52. https://doi.org/10.1007/s00425-015-2285-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1007/s00425-015-2285-5
dc.description.upvformatpinicio 39 es_ES
dc.description.upvformatpfin 52 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 242 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 291386 es_ES
dc.identifier.eissn 1432-2048
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Molecular Biology Organization es_ES
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