- -

A dominant-negative form of Arabidopsis AP-3 #-adaptin improves intracellular pH homeostasis.

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

A dominant-negative form of Arabidopsis AP-3 #-adaptin improves intracellular pH homeostasis.

Show simple item record

Files in this item

dc.contributor.author Niñoles Rodenes, Regina es_ES
dc.contributor.author Rubio, Lourdes es_ES
dc.contributor.author García Sánchez, María J. es_ES
dc.contributor.author Fernandez, Jose A. es_ES
dc.contributor.author Bueso Ródenas, Eduardo es_ES
dc.contributor.author Alejandro Martínez, Santiago es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.date.accessioned 2016-09-05T08:40:35Z
dc.date.available 2016-09-05T08:40:35Z
dc.date.issued 2013-05
dc.identifier.issn 0960-7412
dc.identifier.uri http://hdl.handle.net/10251/68694
dc.description.abstract [EN] Intracellular pH (pHi) is a crucial parameter in cellular physiology but its mechanisms of homeostasis are only partially understood. To uncover novel roles and participants of the pHi regulatory system, we have screened an Arabidopsis mutant collection for resistance of seed germination to intracellular acidification induced by weak organic acids (acetic, propionic, sorbic). The phenotypes of one identified mutant, weak acid-tolerant 1-1D (wat1-1D) are due to the expression of a truncated form of AP-3 -adaptin (encoded by the PAT2 gene) that behaves as a as dominant-negative. During acetic acid treatment the root epidermal cells of the mutant maintain a higher pHi and a more depolarized plasma membrane electrical potential than wild-type cells. Additional phenotypes of wat1-1D roots include increased rates of acetate efflux, K+ uptake and H+ efflux, the latter reflecting the in vivo activity of the plasma membrane H+-ATPase. The in vitro activity of the enzyme was not increased but, as the H+-ATPase is electrogenic, the increased ion permeability would allow a higher rate of H+ efflux. The AP-3 adaptor complex is involved in traffic from Golgi to vacuoles but its function in plants is not much known. The phenotypes of the wat1-1D mutant can be explained if loss of function of the AP-3 -adaptin causes activation of channels or transporters for organic anions (acetate) and for K+ at the plasma membrane, perhaps through miss-localization of tonoplast proteins. This suggests a role of this adaptin in trafficking of ion channels or transporters to the tonoplast. es_ES
dc.description.sponsorship This work was supported by grants BFU2008-00604 from the 'Ministerio de Ciencia e Innovacion' (Madrid, Spain) and from PROME-TEO/2010/038 of the 'Conselleria de Educacion' (Valencia, Spain). We thank Frans J. Maathuis (York, United Kingdom) for the pA7-TPK1-GFP plasmid, Enrico Martinoia (Zurich, Switzerland) for the pART7-ALMT9-GFP plasmid and for the tdt mutant, Karin Schumacher (Heidelberg, Germany) for the vha-a2 vha-a3 mutant, Jose M. Pardo (Sevilla, Spain) for the nhx2-1 mutant, NASC for the akt1-1 mutant, Roberto A. Gaxiola (Tempe, Arizona) for the AVP1 over-expression line and Wei-Hua Wu (Beijing, China) for the AKT1 over-expression line. R. Ninoles was supported by a JAE-preDOC contract ('Consejo Superior de Investigaciones Cientificas', Madrid, Spain). en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation Ministerio de Ciencia e Innovacion (Madrid, Spain) BFU2008-00604 es_ES
dc.relation Conselleria de Educacion (Valencia, Spain) PROMETEO/2010/038 es_ES
dc.relation JAE-preDOC contract (Consejo Superior de Investigaciones Cientificas, Madrid, Spain) es_ES
dc.relation.ispartof Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject H plus -ATPase es_ES
dc.subject Membrane potential es_ES
dc.subject Ion channels es_ES
dc.subject Acetic acid es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A dominant-negative form of Arabidopsis AP-3 #-adaptin improves intracellular pH homeostasis. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/tpj.12138
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Niñoles Rodenes, R.; Rubio, L.; García Sánchez, MJ.; Fernandez, JA.; Bueso Rodenas, E.; Alejandro Martinez, S.; Serrano Salom, R. (2013). A dominant-negative form of Arabidopsis AP-3 #-adaptin improves intracellular pH homeostasis. Plant Journal. 74(4):557-568. doi:10.1111/tpj.12138 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/tpj.12138 es_ES
dc.description.upvformatpinicio 557 es_ES
dc.description.upvformatpfin 568 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 74 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 255509 es_ES
dc.identifier.eissn 1365-313X


This item appears in the following Collection(s)

Show simple item record