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Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc

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Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc

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Santiago Felipe, S.; Tortajada-Genaro, LA.; Puchades, R.; Maquieira Catala, Á. (2016). Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc. Microchimica Acta. 183(3):1195-1202. doi:10.1007/s00604-016-1745-3

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Title: Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc
Author: Santiago Felipe, Sara Tortajada-Genaro, Luis Antonio Puchades, Rosa Maquieira Catala, Ángel
UPV Unit: Universitat Politècnica de València. Departamento de Química - Departament de Química
Issued date:
Abstract:
An integrated method for the parallelized detection of multiple DNA target sequences is presented by using microstructures in a digital versatile disc (DVD). Samples and reagents were managed by using both the capillary ...[+]
Subjects: Multiplex PCR , DNA , Recombinase polymerase amplification (RPA) , Compact disc , High-throughput screening , Food safety
Copyrigths: Reserva de todos los derechos
Source:
Microchimica Acta. (issn: 0026-3672 ) (eissn: 1436-5073 )
DOI: 10.1007/s00604-016-1745-3
Publisher:
Springer Verlag (Germany)
Publisher version: https://dx.doi.org/10.1007/s00604-016-1745-3
Thanks:
This research has been funded through projects FEDER PrometeoII/2014/040 (GVA), and CTQ/2013/45875-R (MINECO). The Spanish Ministry of Education and Science provided S.S.F. with a grant for PhD studies.
Type: Artículo

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