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Detection of food-borne pathogens with DNA arrays on disk

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Detection of food-borne pathogens with DNA arrays on disk

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dc.contributor.author Arnandis Chover, Tania es_ES
dc.contributor.author Morais, Sergi es_ES
dc.contributor.author Tortajada-Genaro, Luis Antonio es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author Berganza, J. es_ES
dc.contributor.author Olabarria, G. es_ES
dc.date.accessioned 2016-09-12T10:48:04Z
dc.date.available 2016-09-12T10:48:04Z
dc.date.issued 2012
dc.identifier.issn 0039-9140
dc.identifier.uri http://hdl.handle.net/10251/69258
dc.description.abstract A DNA oligonucleotide array for duplex pathogen detection on a DVD platform is developed. The assay involves hybridization of PCR products and optical detection using compact disc technology. Different DNA array constructions for attachment of synthetic oligonucleotides on to DVD surface are evaluated, finding that streptavidin-biotin coupling method yielded the highest sensitivity in combination with enzymatic signal amplification. Issues of importance for the DNA array construction such immobilized probes design, PCR product labeling strategy and composition of the hybridization buffer were addressed. The methodology was proved scoring single nucleotide polymorphisms with high selectivity. The assay capability was also demonstrated by the identification of two pathogenic microorganisms in powder milk samples. In fifty minutes, the DVD-array system identifies Salmonella spp. and Cronobacter spp. (previously named Enterobacter sakazakii) precise and simultaneously with a sensitivity of 100 and 102 cfu/mL, respectively, in infant milk. Results were in good agreement with those obtained by quantitative real-time PCR. © 2012 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship This work was funded by the projects PATSENS, PSE-010000-2008-6 (Spanish Government and EU FEDER funds), FEDER CTQ2010-15943 (CICYT, Spain), PROMETEO 2010/008 and ACOMP/2012/158 (Generalitat Valenciana). The Spanish MEC provided T.A-C with a grant for her PhD studies. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Spanish Government PSE-010000-2008-6 es_ES
dc.relation EU FEDER funds es_ES
dc.relation CICYT, Spain FEDER CTQ2010-15943 es_ES
dc.relation Generalitat Valenciana PROMETEO 2010/008 ACOMP/2012/158 es_ES
dc.relation Spanish MEC es_ES
dc.relation.ispartof Talanta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Discs es_ES
dc.subject DNA array es_ES
dc.subject Food-borne pathogens es_ES
dc.subject Optical detection es_ES
dc.subject Compact disc technologies es_ES
dc.subject DNA oligonucleotides es_ES
dc.subject Enterobacter es_ES
dc.subject High selectivity es_ES
dc.subject Milk sample es_ES
dc.subject Pathogen detection es_ES
dc.subject Pathogenic microorganisms es_ES
dc.subject PCR products es_ES
dc.subject Quantitative real time PCR es_ES
dc.subject Salmonella spp es_ES
dc.subject Signal amplifications es_ES
dc.subject Single nucleotide polymorphisms es_ES
dc.subject Streptavidin-biotin coupling es_ES
dc.subject Data storage equipment es_ES
dc.subject DNA es_ES
dc.subject Oligonucleotides es_ES
dc.subject Pathogens es_ES
dc.subject Product design es_ES
dc.subject Proteins es_ES
dc.subject Signal processing es_ES
dc.subject Videodisks es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Detection of food-borne pathogens with DNA arrays on disk es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.talanta.2012.09.049
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Arnandis Chover, T.; Morais, S.; Tortajada-Genaro, LA.; Puchades, R.; Maquieira Catala, Á.; Berganza, J.; Olabarria, G. (2012). Detection of food-borne pathogens with DNA arrays on disk. Talanta. 101:405-412. doi:10.1016/j.talanta.2012.09.049 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.talanta.2012.09.049 es_ES
dc.description.upvformatpinicio 405 es_ES
dc.description.upvformatpfin 412 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 101 es_ES
dc.relation.senia 232761 es_ES


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