- -

Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors

Show simple item record

Files in this item

dc.contributor.author Santiago Felipe, Sara es_ES
dc.contributor.author Tortajada-Genaro, Luis Antonio es_ES
dc.contributor.author Carrascosa Rubio, Javier es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2016-09-12T12:26:49Z
dc.date.available 2016-09-12T12:26:49Z
dc.date.issued 2016-05-15
dc.identifier.issn 0956-5663
dc.identifier.uri http://hdl.handle.net/10251/69265
dc.description.abstract An integrated device composed of micro-reactors embedded onto compact discs is proposed for real-time targeted DNA determination. The method principle is based on in-disc loop-mediated isothermal amplification (iD-LAMP) and quantitative optical read-out by a disc drive. In the presence of a target, the turbidimetric or colorimetric properties of reaction solution change, and the transmitted intensity of the disc drive laser modifies according to reaction yield. Monitoring real-time curves allowed the quantitative determination of DNA template amounts. The best amplification/detection results were obtained with micro-reactors (2mm diameter and 1.1mm in depth) drilled on a digital video disc (DVD) and detection based on the colorimetric mode. As proof-of-concept, the assay was applied to detect pathogenic bacteria Salmonella spp. and to identify bovine meat in food samples. Ninety-six samples were simultaneously analysed in 15min, with high selectivity and sensitivity (5CFU/mL and 10µg/g for bacteria and meat, respectively). The in-disc results were comparable to those obtained by conventional LAMP or qPCR approaches. The developed device allows low sample and reagent consumption (3µL of reaction), portability, ease-of-use, and rapid low-cost high-throughput analyses. es_ES
dc.description.sponsorship This research has been funded through projects FEDER-CTQ/2013/45875-R (MINECO) and Prometeo II/2014/040 (GVA). The Spanish Ministry of Education and Science provided S.S.F. with a grant AP2009-4518 for her PhD studies. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MINECO FEDER-CTQ/2013/45875-R es_ES
dc.relation Prometeo (GVA) II/2014/040 es_ES
dc.relation Spanish Ministry of Education and Science AP2009-4518 es_ES
dc.relation.ispartof Biosensors and Bioelectronics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject DNA amplification es_ES
dc.subject LAMP es_ES
dc.subject Isothermal real-time es_ES
dc.subject Micro-reactors es_ES
dc.subject Compact disc es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bios.2015.12.045
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Santiago Felipe, S.; Tortajada-Genaro, LA.; Carrascosa Rubio, J.; Puchades, R.; Maquieira Catala, Á. (2016). Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors. Biosensors and Bioelectronics. 79:300-306. doi:10.1016/j.bios.2015.12.045 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.bios.2015.12.045 es_ES
dc.description.upvformatpinicio 300 es_ES
dc.description.upvformatpfin 306 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 79 es_ES
dc.relation.senia 299926 es_ES
dc.identifier.eissn 1873-4235


This item appears in the following Collection(s)

Show simple item record