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dc.contributor.author | Rodrigo Tarrega, Guillermo | es_ES |
dc.contributor.author | Majer, Eszter | es_ES |
dc.contributor.author | Prakash, Satya | es_ES |
dc.contributor.author | Daros Arnau, Jose Antonio | es_ES |
dc.contributor.author | Jaramillo, Alfonso | es_ES |
dc.contributor.author | Poyatos, Juan F. | es_ES |
dc.date.accessioned | 2016-09-15T10:28:00Z | |
dc.date.available | 2016-09-15T10:28:00Z | |
dc.date.issued | 2015-09-01 | |
dc.identifier.issn | 0006-3495 | |
dc.identifier.uri | http://hdl.handle.net/10251/69697 | |
dc.description.abstract | [EN] The regulation of gene expression, triggered by conformational changes in RNA molecules, is widely observed in cellular systems. Here, we examine this mode of control by means of a model-based design and construction of a fully synthetic riboregulatory device. We present a theoretical framework that rests on a simple energy model to predict the dynamic response of such a system. Following an equilibrium description, our framework integrates thermodynamic properties-anticipated with an RNA physicochemical model-with a detailed description of the intermolecular interaction. The theoretical calculations are confirmed with an experimental characterization of the action of the riboregulatory device within living cells. This illustrates, more broadly, the predictability of genetic robustness on synthetic systems, and the faculty to engineer gene expression programs from a minimal set of first principles. | es_ES |
dc.description.sponsorship | This work was supported by the AXA Research Fund and the CSIC Intramural grant No. 201440I017 to G.R., the Spanish Ministry of Education, Culture and Sports FPU fellowship AP2012-3751 to E.M., the Spanish Ministry of Economy and Competitiveness grants No. AGL2013-49919-EXP and No. BIO2011-26741 to J.-A.D. and No. BFU2011-24691 to J.F.P., and the European Union grant No. FP7-KBBE-613745 (Programming synthetic networks for bio-based production of value chemicals) to A.J. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Biophysical Society | es_ES |
dc.relation.ispartof | Biophysical Journal | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Small RNA | es_ES |
dc.subject | Automated design | es_ES |
dc.subject | Gene-expression | es_ES |
dc.subject | Noncoding Rnas | es_ES |
dc.subject | Transcriptional Regulation | es_ES |
dc.subject | Protein Expression | es_ES |
dc.subject | Escherichia-Coli | es_ES |
dc.subject | Regulatory Rnas | es_ES |
dc.subject | Bacteria | es_ES |
dc.subject | Biology | es_ES |
dc.title | Exploring the Dynamics and Mutational Landscape of Riboregulation with a Minimal Synthetic Circuit in Living Cells | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.bpj.2015.07.021 | |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/613745/EU/Programming synthetic networks for bio-based production of value chemicals/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CSIC//201440I017/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MECD//AP2012-3751/ES/AP2012-3751/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2013-49919-EXP/ES/DETECCION DE PATOGENOS Y BIOCOMPUTACION MEDIANTE CIRCUITOS REGULADORES EN PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2011-26741/ES/PATOGENOS DE RNA DE PLANTAS: INTERACCION CON EL HUESPED Y DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2011-24691/ES/PLASTICIDAD FENOTIPICA EN MICROORGANISMOS: CAUSAS Y CONSECUENCIAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Rodrigo Tarrega, G.; Majer, E.; Prakash, S.; Daros Arnau, JA.; Jaramillo, A.; Poyatos, JF. (2015). Exploring the Dynamics and Mutational Landscape of Riboregulation with a Minimal Synthetic Circuit in Living Cells. Biophysical Journal. 109(5):1070-1076. https://doi.org/10.1016/j.bpj.2015.07.021 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.bpj.2015.07.021 | es_ES |
dc.description.upvformatpinicio | 1070 | es_ES |
dc.description.upvformatpfin | 1076 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 109 | es_ES |
dc.description.issue | 5 | es_ES |
dc.relation.senia | 305724 | es_ES |
dc.identifier.pmid | 26331264 | en_EN |
dc.identifier.pmcid | PMC4564674 | en_EN |
dc.contributor.funder | European Commission | |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte | |
dc.contributor.funder | Ministerio de Economía y Competitividad | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |