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Identification of Methyl-tiophanate resistance in field strains of Fusicladium eriobotryae, the causal agent of loquat scab

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Identification of Methyl-tiophanate resistance in field strains of Fusicladium eriobotryae, the causal agent of loquat scab

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dc.contributor.author González Domínguez, Elisa es_ES
dc.contributor.author Berbegal Martínez, Mónica es_ES
dc.contributor.author Guillem Amat, Ana es_ES
dc.contributor.author Armengol Fortí, Josep es_ES
dc.contributor.editor R. Lo Bianco, J. Janick es_ES
dc.date.accessioned 2016-09-19T14:46:40Z
dc.date.available 2016-09-19T14:46:40Z
dc.date.issued 2015-08
dc.identifier.isbn 978-94-62610-88-0
dc.identifier.issn 0567-7572
dc.identifier.issn 2406-6168
dc.identifier.uri http://hdl.handle.net/10251/70114
dc.description The original publication is available at www.actahort.org es_ES
dc.description.abstract [EN] Benzimidazoles, and specifically methyl-thiophanate, have been broadly used in the last two decades in Spain to control loquat (Eriobotrya japonica Lind.) scab caused by Fusicladium eriobotryae (Cavara) Sac. However, to date no study has been conducted to identify resistant isolates to this fungicide. For this purpose, 249 isolates of F. eriobotryae collected from the main loquat producing areas in Spain (Andalucía and Comunidad Valenciana) were grown in culture medium amended with methyl-thiophanate (0.5 µg/ml). After one month of incubation, the relative growth compared with that obtained in a non-amended medium (control) was calculated; 33 isolates were resistant to methyl-thiophanate (>30% growth relative to the control). In addition, the resistance to methyl-thiophanate in F. eriobotryae was characterized by mutations in the β-tubulin gene. DNA of representative susceptible and resistant isolates was extracted and a partial sequence of the β-tubulin gene was obtained. Resistant isolates showed the presence of only one non-synonymous mutation in the codons 198, 200 or 240 of this gene, which has been previously reported as characteristic of methyl-thiophanate resistance in other plant pathogens. These mutations could be used to develop a screening protocol to identify isolates of F. eriobotryae resistant to methyl-thiophanate. es_ES
dc.description.sponsorship This work has been funded by Cooperativa Agrícola de Callosa d’En Sarrià (Alicante, Spain). We thank loquat growers, researchers and technicians from the provinces surveyed, who helped us visiting the fields.
dc.format.extent 4 es_ES
dc.language Inglés es_ES
dc.publisher International Society for Horticultural Science (ISHS) es_ES
dc.relation.ispartof Acta Horticulturae es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Benzimidazoles es_ES
dc.subject Eriobotrya japonica es_ES
dc.subject Fungicide resistance es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification ECOSISTEMAS AGROFORESTALES (UPV) es_ES
dc.title Identification of Methyl-tiophanate resistance in field strains of Fusicladium eriobotryae, the causal agent of loquat scab es_ES
dc.type Artículo es_ES
dc.type Comunicación en congreso es_ES
dc.identifier.doi 10.17660/ActaHortic.2015.1092.43
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation González Domínguez, E.; Berbegal Martinez, M.; Guillem Amat, A.; Armengol Fortí, J. (2015). Identification of Methyl-tiophanate resistance in field strains of Fusicladium eriobotryae, the causal agent of loquat scab. Acta Horticulturae. (1092):289-292. doi:10.17660/ActaHortic.2015.1092.43 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.conferencename IV International Symposium on Loquat es_ES
dc.relation.conferencedate May 12-15, 2014 es_ES
dc.relation.conferenceplace Palermo, Italy es_ES
dc.relation.publisherversion http://dx.doi.org/10.17660/ActaHortic.2015.1092.43 es_ES
dc.description.upvformatpinicio 289 es_ES
dc.description.upvformatpfin 292 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.issue 1092 es_ES
dc.relation.senia 294761 es_ES


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