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Changes in xyloglucan endotransglucosylase/hydrolase (XTHs) expression and XET activity during apple fruit infection by Penicillium expansum Link. A

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Changes in xyloglucan endotransglucosylase/hydrolase (XTHs) expression and XET activity during apple fruit infection by Penicillium expansum Link. A

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dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Lorences, E.P. es_ES
dc.date.accessioned 2016-09-28T07:20:49Z
dc.date.available 2016-09-28T07:20:49Z
dc.date.issued 2014-02
dc.identifier.issn 0929-1873
dc.identifier.uri http://hdl.handle.net/10251/70536
dc.description.abstract Although the plant cell wall is an extremely effective physical barrier against attack by pathogens, most phytopathogenic microorganisms produce enzymes that are capable of degrading cell wall polymers such as pectin and hemicelluloses. In this work we investigate the possible implication of the cell wall enzyme XET in the host-pathogen interaction as well as the XTH gene regulation during apple fruit infection by Penicillium expansum Link. A. We characterized the time course of fungal infection in apple fruit, and the XET activity and the expression of eleven MdXTHs. The results showed an important decrease in XET specific activity in the fruits after 24, 48 and 72 h of fruit infection (42.9 %, 73.1 % and 95.1 % respectively as compared with the controls). The expression analysis of the MdXTHs showed an important decrease in expression, as the infection progressed, in MdXTH2 and MdXTH10, as well as in MdXTH3, MdXTH4 and MdXTH5, but mainly after 72 h of fruit infection; and in MdXTH1, and MdXTH9 after 24 and 48 h of infection. These results suggested that the decrease in XET activity during infection could be mainly attributed to the decrease in expression of MdXTH2 and MdXTH10, since those were the MdXTHs that showed the highest expression levels during ripening, particularly MdXTH10. These results support the suggestion that inhibition of MdXTHs expression, and consequently the decrease in XET specific activity, could represent a pathogenicity factor, since the potential reconstructing role of the enzyme also decreased and the infection could progress. es_ES
dc.description.sponsorship This work was funded by GVA, PROMETEO/2009/075. We wish to thank Mr D. A. Lindsay for correcting the English version of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cell wall es_ES
dc.subject Fruit infection es_ES
dc.subject Malus domestica es_ES
dc.subject Penicillium expansum es_ES
dc.subject Transglucosylation es_ES
dc.subject Xyloglucan es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Changes in xyloglucan endotransglucosylase/hydrolase (XTHs) expression and XET activity during apple fruit infection by Penicillium expansum Link. A es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-013-0327-z
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO09%2F2009%2F075/ES/Mejora de plantas con interés agronómico y forestal- MEPIAF/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Muñoz Bertomeu, J.; Lorences, E. (2014). Changes in xyloglucan endotransglucosylase/hydrolase (XTHs) expression and XET activity during apple fruit infection by Penicillium expansum Link. A. European Journal of Plant Pathology. 138(2):273-282. https://doi.org/10.1007/s10658-013-0327-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10658-013-0327-z es_ES
dc.description.upvformatpinicio 273 es_ES
dc.description.upvformatpfin 282 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 138 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 255040 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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