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Detection, characterization and host range studies of Pepino mosaic virus in Cyprus

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Detection, characterization and host range studies of Pepino mosaic virus in Cyprus

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dc.contributor.author Papayiannis, L. es_ES
dc.contributor.author Kokkinos, C.D. es_ES
dc.contributor.author Alfaro Fernández, Ana Olvido es_ES
dc.date.accessioned 2016-09-28T07:21:54Z
dc.date.available 2016-09-28T07:21:54Z
dc.date.issued 2012-01
dc.identifier.issn 0929-1873
dc.identifier.uri http://hdl.handle.net/10251/70537
dc.description.abstract [EN] Pepino mosaic virus (PepMV, Genus Potexvirus, Family Flexiviridae) is a mechanically transmitted viral disease that has emerged as a significant problem of greenhouse tomato crops in Europe and around the world. Although previous studies in Cyprus suggested that the virus was not present on the island, in 2009 tomato fruits from two major tomato production areas exhibited symptoms of yellow mosaic and discolouration, similar to those induced by PepMV. Consequently, an extensive survey was conducted in all tomato producing areas of the country to identify the incidence and prevalence of PepMV in protected and open field tomato crops. Analysis of 3500 leaf samples from tomato plants and weeds with DAS-ELISA and real-time RT-PCR showed that PepMV was present in all tomato growing areas of the island. The virus was detected in both protected and open field tomato plants, as well as in 20 weed species in the families of Amaranthaceae, Chenopodiaceae, Compositae, Convolvulaceae, Malvaceae, Plantaginaceae and Solanaceae. All Cypriot isolates assayed belonged to the CH2 genotype. Biological assays with two Cypriot isolates showed that they could infect cultivated and weed species including Vigna unguiculata, Solanum melongena, Nicotiana tabacum, Malva parviflora, Sonchus oleraceus, Solanum nigrum, Convolvulus arvensis, Chrysanthemum segetum and Calendula arvensis. To our knowledge, this is the first study to report Chrysanthemum segetum and Calendula arvensis as hosts of PepMV. es_ES
dc.description.sponsorship This work was funded by a grant from the Cyprus Research Promotion Foundation and supported by the European Commission in the 6th Framework Programme (PEPEIRA EC contract No 044189). The authors thank Dr. R. van der Vlugt (Plant Research International, The Netherlands), Prof. M. Ravnikar and Dr. I. Gutierrez-Aguirre (National Institute of Biology, Slovenia), Prof. C. Jorda (Instituto Agroforestal Mediterraneao, Valencia, Spain), for providing Pepino mosaic virus isolates used as positive controls. The authors would also like to thank Drs. G. Neophytou, A. Melifronidou, D. Koudounas (Cyprus Department of Agriculture) and I. Harkou and Y. Markou for their valuable assistance in surveys and laboratory experiments and G. Economides for identification of weed species. Dr. M. Stavrinides and Dr. A. Kyriakou are acknowledged for critically reviewing this manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Genotype es_ES
dc.subject PepMV es_ES
dc.subject Potexvirus es_ES
dc.subject Real-Time RT-PCR es_ES
dc.subject Weed hosts es_ES
dc.title Detection, characterization and host range studies of Pepino mosaic virus in Cyprus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-011-9854-7
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/044189/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Papayiannis, L.; Kokkinos, C.; Alfaro Fernández, AO. (2012). Detection, characterization and host range studies of Pepino mosaic virus in Cyprus. European Journal of Plant Pathology. 132(1):1-7. https://doi.org/10.1007/s10658-011-9854-7 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10658-011-9854-7 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 7 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 132 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 235545 es_ES
dc.contributor.funder European Commission
dc.contributor.funder Research Promotion Foundation es_ES
dc.description.references Alfaro-Fernández, A., Cebrián, M. C., Córdoba-Sellés, M. C., Herrera-Vásquez, J. A., & Jordá, C. (2008). First report of the US1 strain of Pepino mosaic virus in tomato in the Canary Isands, Spain. Plant Disease, 92, 1590. es_ES
dc.description.references Alfaro-Fernández, A., Sánchez-Navarro, J. A., Cebrián, M. C., Córdoba-Sellés, M. C., Pallás, V., & Jordá, C. (2009). Simultaneous detection and identification of Pepino mosaic virus (PepMV) isolates by multiplex one-step RT-PCR. European Journal of Plant Pathology, 125, 143–158. es_ES
dc.description.references Campanella, J. J., Bitincka, L., & Smalley, J. (2003). MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics, 4, 29–33. es_ES
dc.description.references Córdoba, M. C., Martínez-Priego, L., & Jordá, C. (2004). New natural hosts of Pepino mosaic virus in Spain. Plant Disease, 88, 906. es_ES
dc.description.references Córdoba-Sellés, M. C., García-Rández, A., Alfaro-Fernández, A., & Jordá, C. (2007). Seed transmission of Pepino mosaic virus and efficacy of tomato seed disinfection treatments. Plant Disease, 91, 1250–1254. es_ES
dc.description.references Gómez, P., Sempere, R. N., Elena, S. F., & Aranda, M. (2009). Mixed infections of Pepino mosaic virus strains modulate the evolutionary dinamics of this emerging virus. Journal of Virology, 83, 12378–12387. es_ES
dc.description.references Gutiérrez-Aguirre, I., Mehle, N., Delić, D., Gruden, K., Mumford, R., & Ravnikar, M. (2009). Real-time quantitative PCR based sensitive detection and genotype discrimination of Pepino mosaic virus. Journal of Virological Methods, 162, 46–55. es_ES
dc.description.references Hanssen, I. M., & Thomma, B. P. H. J. (2010). Pepino mosaic virus: a successful pathogen that rapidly evolved from emerging to endemic in tomato crops. Molecular Plant Pathology, 11, 179–189. es_ES
dc.description.references Hanssen, I. M., Mumford, R., Blystad, D.-R., Cortez, I., Hasiów-Jaroszewska, B., Hristova, D., et al. (2009). Seed transmission of Pepino mosaic virus in tomato. European Journal of Plant Pathology, 126, 145–152. es_ES
dc.description.references Jones, R. A. C., Koening, R., & Lesemann, D. E. (1980). Pepino mosaic virus, a new Potexvirus from pepino (Solanum muricatum). Annals of Applied Biology, 94, 61–68. es_ES
dc.description.references Jordá, C., Lázaro-Pérez, A., Martínez-Culebras, P., & Lacasa, A. (2001). First report of Pepino mosaic virus on natural hosts. Plant Disease, 85, 1292. es_ES
dc.description.references Jordá, C., Lázaro-Pérez, A., Martínez-Culebras, P., & Abad, P. (2001). First report of Pepino mosaic virus on tomato in Spain. Plant Disease, 85, 1292. es_ES
dc.description.references Kumar, S., Tamura, K., & Nei, M. (2004). MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformatic, 5, 150–163. es_ES
dc.description.references Ling, K. S., Wechter, W. P., & Jordan, R. (2007). Development of a one-step immunocapture real-time TaqMan RT-PCR assay for the broad spectrum detection of Pepino mosaic virus. Journal of Virological Methods, 144, 65–72. es_ES
dc.description.references Mumford, R. A., & Metcalfe, E. J. (2001). The partial sequencing of the genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with othes isolates from Europe and Peru. Archives of Virology, 146, 2455–2460. es_ES
dc.description.references Pagán, I., Córdoba-Sellés, M. C., Martínez-Priego, L., Fraile, A., Malpica, J. M., Jordá, C., et al. (2006). Genetic structure of the population of Pepino mosaic virus infecting tomato crops in Spain. Phytopathology, 96, 274–279. es_ES
dc.description.references Papayiannis, L. C., Savvides, A., Chatziafksentis, K., Kapari-Isaia, T., Dovas, C., Katis, N. I., et al. (2008). Incidence of viruses infecting tomato crops in Cyprus. Phytopathologia Mediterranea, 47, 159. es_ES
dc.description.references Pospieszny, H., Hásiow, B., & Borodynko, N. (2008). Characterization of two distinct Polish isolates of Pepino mosaic virus. European Journal of Pathology, 122, 443–445. es_ES
dc.description.references Spence, N. J., Basham, J., Mumford, R. A., Hayman, G., Edmondson, R., & Jones, D. R. (2006). Effect of Pepino mosaic virus on the yield and quality of glasshouse-grown tomatoes in the UK. Plant Pathology, 55, 595–606. es_ES
dc.description.references Soler, S., Prohens, J., Diez, M. J., & Nuez, E. (2002). Natural occurrence of Pepino mosaic virus in Lycopersicon species in Central and Southern Peru. Journal of Phytopathology, 150, 45–53. es_ES
dc.description.references Van der Vlugt, R. A. A. (2009). Pepino mosaic virus. Hellenic Plant Protection Journal, 2, 47–56. es_ES
dc.description.references Van der Vlugt, R. A. A., Stijger, C. C. M. M., Verhoeven, J. Th. J., & Leserman, D. E. (2000). First report of Pepino mosaic virus on tomato. Plant Disease, 84, 103. es_ES
dc.description.references Van der Vlugt, R. A. A., Cuperus, C., Vink, J., Stijger, C. C. M. M., Lesemann, D.-E., Verhoeven, J. Th. J., et al. (2002). Identification and characterisation of Pepino mosaic potex virus in tomato. Bulletin OEPP/EPPO Bulletin, 32, 503–508. es_ES
dc.description.references Verhoeven, J. T. J., Van der Vlugt, R. A. A., & Roenhorst, J. W. (2003). High similarity between tomato isolates of Pepino mosaic virus suggest a common origin. European Journal of Plant Pathology, 109, 419–425. es_ES
dc.description.references Wright, D., & Mumford, R. (1999). Pepino mosaic potexvirus (PepMV). First records in tomato in United Kingdom. Central Science Laboratory, York, UK. Plant Disease Notice, 89. es_ES


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