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Simultaneous detection of eight viruses and two viroids affecting stone fruit trees by using a unique polyprobe

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Simultaneous detection of eight viruses and two viroids affecting stone fruit trees by using a unique polyprobe

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dc.contributor.author Peiró Morell, Ana es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Sanchez Navarro, Jesus Angel es_ES
dc.date.accessioned 2016-09-28T08:22:09Z
dc.date.available 2016-09-28T08:22:09Z
dc.date.issued 2012-04
dc.identifier.issn 0929-1873
dc.identifier.uri http://hdl.handle.net/10251/70553
dc.description.abstract The use of riboprobes carrying partial sequences of different plant viruses or viroids fused in tandem, has permitted the simultaneous detection of up to six different pathogens using a non-radioactive molecular hybridization procedure. In the present report, we describe the development of a unique polyprobe (poly10) with the capacity to detect viruses and viroids commonly found infecting fruit trees. The poly10 covers eight viruses: Apple mosaic virus (ApMV), Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), American plum line pattern (APLPV), Plum pox virus (PPV), Apple chlorotic leaf spot virus (ACLSV), Apricot latent virus (ApLV), Plum bark necrosis and stem pitting-associated virus (PBNSPaV) and two viroids: Hop stunt viroid (HSVd) and Peach latent mosaic viroid (PLMVd). Poly10 is comparable to the individual riboprobes in terms of end-point dilution limit and specificity, allowing the detection of up to 2.5 picograms of viral or viroidal RNA. However, the polyprobe requires a hybridization temperature of 60 degrees C instead of the standard 68 degrees C. The validation of the new simultaneous detection strategy was confirmed by the analysis of 60 field samples, which came from seven different hosts. The use of the polyprobe as an alternative to other routinely used detection methods is discussed. es_ES
dc.description.sponsorship A. P. M. is the recipient of a JAE-Pre contract from the Consejo Superior de Investigaciones Cientificas (CSIC). We thank L. Corachan for her excellent technical assistance. This work was supported by grant BIO2008-03528 from the Spanish granting agency DGICYT and by grant ACOMP/2010/214 from the Generalitat Valenciana. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Consejo Superior de Investigaciones Cientificas (CSIC) es_ES
dc.relation Spanish granting agency DGICYT BIO2008-03528 es_ES
dc.relation Generalitat Valenciana ACOMP/2010/214 es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Multiplex es_ES
dc.subject Non-radioactive molecular hybridization es_ES
dc.subject Stone fruit tree viruses and viroids es_ES
dc.subject Dig-RNA probe es_ES
dc.title Simultaneous detection of eight viruses and two viroids affecting stone fruit trees by using a unique polyprobe es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-011-9893-0
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Peiró Morell, A.; Pallás Benet, V.; Sanchez Navarro, JA. (2012). Simultaneous detection of eight viruses and two viroids affecting stone fruit trees by using a unique polyprobe. European Journal of Plant Pathology. 132(4):469-475. doi:10.1007/s10658-011-9893-0 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10658-011-9893-0 es_ES
dc.description.upvformatpinicio 469 es_ES
dc.description.upvformatpfin 475 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 132 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 233151 es_ES


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