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DESIGN OF A MICRO-FLUIDIC DEVICE FOR THE ANALYSIS OF PROTEINS EXPRESSION VARIABILITY

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DESIGN OF A MICRO-FLUIDIC DEVICE FOR THE ANALYSIS OF PROTEINS EXPRESSION VARIABILITY

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dc.contributor.advisor Navarro Herrero, José Luís es_ES
dc.contributor.advisor Picó Marco, Jesús Andrés es_ES
dc.contributor.author Arco Sola, Javier es_ES
dc.date.accessioned 2016-10-04T09:52:02Z
dc.date.available 2016-10-04T09:52:02Z
dc.date.created 2016-09-26
dc.date.issued 2016-10-04 es_ES
dc.identifier.uri http://hdl.handle.net/10251/71096
dc.description.abstract [EN] The recent progress in micro fabrication methods has provided an excellent tool and a change of paradigm on single cell research. Microfluidic devices allow unprecedented experimental approaches with robust control of the environmental variables, rapid prototyping, flexibility and affordable costs. In this study, the benefits of microfluidic technology are exploited in the field of synthetic biology. A bibliographic review of current technologies leads to the design of a device with the purpose of studying the effect of quorum sensing communication mechanism in protein expression variability. Experimental requirements are analysed in order to provide a functional tool for laboratory research. The main design challenge consist on the trapping mechanism for E. Coli cells due to a single cell volume of around 1.3 µm3 , which approaches the limits of manufacturable structures. The proposed device, named the microDISA device, is simulated to solve by means of finite elements methods the distribution profiles of velocities, pressures and concentrations to assure convenient operating conditions during experimentation. Simulation is also employed as a tool to iteratively improve the designed geometry and provide useful information of the device before prototyping. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Biología sintética es_ES
dc.subject Análisis de variabilidad es_ES
dc.subject Diseño de experimentos es_ES
dc.subject Microfluídica es_ES
dc.subject.classification INGENIERIA DE SISTEMAS Y AUTOMATICA es_ES
dc.subject.other Máster Universitario en Ingeniería Industrial-Màster Universitari en Enginyeria Industrial es_ES
dc.title DESIGN OF A MICRO-FLUIDIC DEVICE FOR THE ANALYSIS OF PROTEINS EXPRESSION VARIABILITY es_ES
dc.type Tesis de máster es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Arco Sola, J. (2016). DESIGN OF A MICRO-FLUIDIC DEVICE FOR THE ANALYSIS OF PROTEINS EXPRESSION VARIABILITY. http://hdl.handle.net/10251/71096. es_ES
dc.description.accrualMethod TFGM es_ES
dc.relation.pasarela TFGM\46989 es_ES


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