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Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation

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Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation

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dc.contributor.author Rodrigo Navarro, Aleixandre es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Saadeddin Saadeddin, Anas es_ES
dc.contributor.author Garcia, Andres J. es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2016-10-06T09:40:42Z
dc.date.available 2016-10-06T09:40:42Z
dc.date.issued 2014-07-28
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10251/71292
dc.description.abstract Genetically modified Lactococcus lactis, non-pathogenic bacteria expressing the FNIII7-10 fibronectin fragment as a protein membrane have been used to create a living biointerface between synthetic materials and mammalian cells. This FNIII7-10 fragment comprises the RGD and PHSRN sequences of fibronectin to bind a5b1 integrins and triggers signalling for cell adhesion, spreading and differentiation. We used L. lactis strain to colonize material surfaces and produce stable biofilms presenting the FNIII7-10 fragment readily available to cells. Biofilm density is easily tunable and remains stable for several days. Murine C2C12 myoblasts seeded over mature biofilms undergo bipolar alignment and form differentiated myotubes, a process triggered by the FNIII7-10 fragment. This biointerface based on living bacteria can be further modified to express any desired biochemical signal, establishing a new paradigm in biomaterial surface functionalisation for biomedical applications. es_ES
dc.description.sponsorship The support from ERC through HealInSynergy 306990 is acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject LACTOCOCCUS-LACTIS es_ES
dc.subject FIBRONECTIN CONFORMATION es_ES
dc.subject BIOFILM FORMATION es_ES
dc.subject POSITIVE ROLE es_ES
dc.subject ADHESION es_ES
dc.subject HYDROGELS es_ES
dc.subject INTEGRIN es_ES
dc.subject MORPHOGENESIS es_ES
dc.subject SURFACES es_ES
dc.subject BINDING es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/srep05849
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306990/EU/Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor Microenvironments/ en_EN
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Rodrigo Navarro, A.; Rico Tortosa, PM.; Saadeddin Saadeddin, A.; Garcia, AJ.; Salmerón Sánchez, M. (2014). Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation. Scientific Reports. 4:5849-5859. https://doi.org/10.1038/srep05849 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/srep05849 es_ES
dc.description.upvformatpinicio 5849 es_ES
dc.description.upvformatpfin 5859 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 4 es_ES
dc.relation.senia 287838 es_ES
dc.identifier.pmid 25068919 en_EN
dc.identifier.pmcid PMC5376178 en_EN
dc.contributor.funder European Commission
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