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Maternal Exposure to High Temperatures Disrupts OCT4 mRNA Expression of Rabbit Pre-Implantation Embryos and Endometrial Tissue.

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Maternal Exposure to High Temperatures Disrupts OCT4 mRNA Expression of Rabbit Pre-Implantation Embryos and Endometrial Tissue.

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dc.contributor.author Marco Jiménez, Francisco es_ES
dc.contributor.author Naturil Alfonso, Carmen es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Jiménez Trigos, María Estrella es_ES
dc.contributor.author García Diego, Fernando Juan es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2016-10-25T07:45:03Z
dc.date.available 2016-10-25T07:45:03Z
dc.date.issued 2013-06
dc.identifier.issn 0936-6768
dc.identifier.uri http://hdl.handle.net/10251/72705
dc.description.abstract [EN] Contents: We examined the effect of prolonged high heat stress on reproductive performance and its relationship with gene expression in pre-implantation embryos and endometrial tissue. In experiment 1, primiparous rabbit does were divided into two environments: control does (maintained between 14 and 22°C) and heat-treated does housed in a climatic chamber (maintained between 25 and 35°C). Females were reproducing, and the litter size and live born kits were assessed at 2nd and 3rd partum. In heat-treated does, lower litter size (9.7 ± 0.48 and 11.4 ± 0.50) and fewer live born kits (7.2 ± 0.55 and 10.2 ± 0.57) were observed, although similar ovulation rates and numbers of pre-implantation embryos were noted. In experiment 2, after 3rd partum multiparous non-lactating does from each experimental group were used to obtain pre-implantation embryos and endometrial tissue. mRNA transcripts from OCT-4, VEGF, erbB3, Ifn-¿{phonetic}, HSP70 and HSP90 were analysed by real-time qPCR. Higher values of OCT-4 expression were observed in embryos and endometrial tissue in females reproduced under heat conditions. Moreover, elevated temperatures have been shown to up-regulate VEGF in embryos and down-regulate Ifn-¿{phonetic} in endometrial tissue. The findings suggest a deleterious temperature effect on litter size and live born kits as a consequence of variation in gene expression pattern of the pre-implantational embryo and the endometrium associated with proliferation and differentiation and probably with implantation and uterine and foetal development during gestation. © 2012 Blackwell Verlag GmbH. es_ES
dc.description.sponsorship This work was supported by the Spanish Research Projects AGL2008–03274 (CICYT). Carmen Naturil was supported by Generalitat Valenciana research programme (Prometeo 2009/125). Estrella Jimenez was supported by a research grant from the Education Ministry of the Valencian Regional Government (programme VALi+d. ACIF/2010/262). English text version was revised by N. Macowan English Language Service.
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation CICYT/AGL2008–03274 es_ES
dc.relation GV/PROMETEO/2009/125 es_ES
dc.relation GV/VALi+d. ACIF/2010/262 es_ES
dc.relation.ispartof Reproduction in Domestic Animals es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Maternal Exposure to High Temperatures Disrupts OCT4 mRNA Expression of Rabbit Pre-Implantation Embryos and Endometrial Tissue. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/rda.12092
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Marco Jiménez, F.; Naturil Alfonso, C.; Peñaranda, D.; Jiménez Trigos, ME.; García Diego, FJ.; Vicente Antón, JS. (2013). Maternal Exposure to High Temperatures Disrupts OCT4 mRNA Expression of Rabbit Pre-Implantation Embryos and Endometrial Tissue. Reproduction in Domestic Animals. 48(3):429-434. doi:10.1111/rda.12092 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/rda.12092 es_ES
dc.description.upvformatpinicio 429 es_ES
dc.description.upvformatpfin 434 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 48 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 229202 es_ES
dc.identifier.eissn 1439-0531
dc.identifier.pmid 23043275
dc.contributor.funder Comisión Interministerial de Ciencia e Innovación
dc.contributor.funder Generalitat Valenciana


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