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Direct Comparison of the Effects of Slow Freezing and Vitrification on Late Blastocyst Gene Expression, Development, Implantation and Offspring of Rabbit Morulae.

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Direct Comparison of the Effects of Slow Freezing and Vitrification on Late Blastocyst Gene Expression, Development, Implantation and Offspring of Rabbit Morulae.

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dc.contributor.author Saenz de Juano Ribes, María de los Desamparados es_ES
dc.contributor.author Marco Jiménez, Francisco es_ES
dc.contributor.author Viudes De Castro, María Pilar es_ES
dc.contributor.author Lavara García, Raquel es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2016-10-25T11:51:02Z
dc.date.available 2016-10-25T11:51:02Z
dc.date.issued 2014-06
dc.identifier.issn 0936-6768
dc.identifier.uri http://hdl.handle.net/10251/72735
dc.description.abstract This study aimed to assess the effect of different cryopreservation procedures (slow freezing vs vitrification) on the gene expression in pre-implantation embryos and its implication in post-implantation embryo losses in rabbit. For this purpose, rabbit morulae were recovered at Day 3 of development, frozen or vitrified and transferred to recipients. Then, embryos were recovered on Day 3 post-transfer (Day 6 of development) or kept until the end of gestation. Apart from the gene expression analysis at Day 6, we also studied the pre-implantatory and foetal development ability of both cryopreserved embryo types by evaluating late blastocyst development at Day 6, embryo implantation at Day 11 post-transfer (Day 14 of development) and birth rate. We reported that slow freezing and vitrification have similar effects on embryo developmental ability till Day 6, but the distribution of losses changes during implantation and further development. These similarities at Day 6 of development were also reflected in gene expression patterns, and transcriptome analysis showed no differences between frozen and vitrified embryos. Our results confirm that vitrification provides better implantation and birth rates than slow freezing for rabbit embryos. As both the techniques are commonly used in human assisted reproduction, further experiments must be conducted to clarify the causes that may hinder foetal development and their impact on adulthood. es_ES
dc.description.sponsorship This work was supported by the Valencian Regional Government research programme (Prometeo 2009/125) and the Spanish Research Projects (CICYT AGL2011-29831-C03-01). M. D. Saenz-de-Juano was supported by a research grant from Generalitat Valenciana (Programa VALI+d, ACIF/2011/254). The authors would like to thank Neil Macowan Language Services for revising the English version of the manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation Valencian Regional Government research programme Prometeo 2009/125 es_ES
dc.relation Spanish Research Projects CICYT AGL2011-29831-C03-01 es_ES
dc.relation Generalitat Valenciana (Programa VALI+d) ACIF/2011/254 es_ES
dc.relation.ispartof Reproduction in Domestic Animals es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject MOUSE EMBRYOS es_ES
dc.subject BOVINE EMBRYOS es_ES
dc.subject SURVIVAL RATE es_ES
dc.subject CRYOPRESERVATION es_ES
dc.subject FERTILITY es_ES
dc.subject PROFILES es_ES
dc.subject VITRO es_ES
dc.subject GENOTYPE es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Direct Comparison of the Effects of Slow Freezing and Vitrification on Late Blastocyst Gene Expression, Development, Implantation and Offspring of Rabbit Morulae. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/rda.12320
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Saenz De Juano Ribes, MDLD.; Marco Jiménez, F.; Viudes De Castro, MP.; Lavara García, R.; Vicente Antón, JS. (2014). Direct Comparison of the Effects of Slow Freezing and Vitrification on Late Blastocyst Gene Expression, Development, Implantation and Offspring of Rabbit Morulae. Reproduction in Domestic Animals. 49(3):505-511. doi:10.1111/rda.12320 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/rda.12320 es_ES
dc.description.upvformatpinicio 505 es_ES
dc.description.upvformatpfin 511 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 49 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 266521 es_ES


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