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Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose

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Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose

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dc.contributor.author Parra Vega, Verónica es_ES
dc.contributor.author Corral Martínez, Patricia es_ES
dc.contributor.author Rivas-Sendra, Alba es_ES
dc.contributor.author Seguí-Simarro, Jose M. es_ES
dc.date.accessioned 2016-11-09T09:11:51Z
dc.date.available 2016-11-09T09:11:51Z
dc.date.issued 2015-11-25
dc.identifier.issn 1664-462X
dc.identifier.uri http://hdl.handle.net/10251/73629
dc.description The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2015.01018 es_ES
dc.description.abstract The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this work, we used high pressure freezing and freeze substitution, immunolocalization, confocal and electron microscopy to analyze the structure and composition of the first cell walls formed during conventional Brassica napus microspore embryogenesis, and in cultures treated to alter the intracellular Ca2+ levels. Our results revealed that one of the first signs of embryogenic commitment is the formation of a callose-rich, cellulose-deficient layer beneath the intine (the subintinal layer), and of irregular, incomplete cell walls. In these events, Ca2+ may have a role. We propose that abnormal cell walls are due to a massive callose synthesis and deposition of excreted cytoplasmic material, and the parallel inhibition of cellulose synthesis. These features were absent in pollen-like structures and in microspore-derived embryos, few days after the end of the heat shock, where abnormal cell walls were no longer produced. Together, our results provide an explanation to a series of relevant aspects of microspore embryogenesis including the role of Ca2+ and the occurrence of abnormal cell walls. In addition, our discovery may be the explanation to why nuclear fusions take place during microspore embryogenesis. es_ES
dc.description.sponsorship We want to express our thanks to the staff of the Electron Microscopy Service of Universitat Politecnica de Valencia. Thanks are also due to Dr. Kim Boutilier (PRI Wageningen, The Netherlands) for her help during the stays of VPV and ARS at her lab, to Dr. Samantha Vernhettes (INRA Versailles, France) for her kind gift of S4B staining, and especially to Prof. L. A. Staehelin for his help and friendship during the stay of JMSS at UC Boulder. This work was supported by grants BEST/20081154 from Generalitat Valenciana and AGL2014-55177-R from Spanish MINECO to JMSS. en_EN
dc.language Inglés es_ES
dc.publisher Frontiers Media es_ES
dc.relation Generalitat Valenciana BEST/20081154 es_ES
dc.relation Spanish MINECO AGL2014-55177-R es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Androgenesis es_ES
dc.subject Benzyl alcohol es_ES
dc.subject Caffeine es_ES
dc.subject Doubled haploids es_ES
dc.subject Microspore embryogenesis es_ES
dc.subject Rapeseed es_ES
dc.subject Cell wall carbohydrates. es_ES
dc.subject Electron Microscopy Service of the UPV
dc.subject.classification GENETICA es_ES
dc.title Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2015.01018
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Parra Vega, V.; Corral Martínez, P.; Rivas-Sendra, A.; Seguí-Simarro, JM. (2015). Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose. Frontiers in Plant Science. 6(1018):1-17. doi:10.3389/fpls.2015.01018 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3389/fpls.2015.01018 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 1018 es_ES
dc.relation.senia 296705 es_ES
dc.identifier.pmcid PMC4658426 en_EN


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