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Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

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Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

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dc.contributor.author Juárez Ortega, Paloma es_ES
dc.contributor.author Presa Castro, Silvia es_ES
dc.contributor.author Espi, Joaquin es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author Antón Martínez, María Teresa es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Buesa, Javier es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2016-11-09T11:20:54Z
dc.date.available 2016-11-09T11:20:54Z
dc.date.issued 2012-04
dc.identifier.issn 1467-7644
dc.identifier.uri http://hdl.handle.net/10251/73650
dc.description.abstract [EN] Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 +/- 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and strongly inhibited virus infection in an in vitro virus neutralization assay. In order to make tomatoes expressing hIgA_2A1 easily distinguishable from wild-type tomatoes, lines expressing hIgA_2A1 transgenes were sexually crossed with a transgenic tomato line expressing the genes encoding Antirrhinum majus Rosea1 and Delila transcription factors, which confer purple colour to the fruit. Consequently, transgenically labelled purple tomato fruits expressing hIgA_2A1 have been developed. The resulting purple-coloured extracts from these fruits contain high levels of recombinant anti-rotavirus neutralizing human IgA in combination with increased amounts of health-promoting anthocyanins. es_ES
dc.description.sponsorship We want to thank Prof. Cathie Martin and Dr. Eugenio Butelli for kindly providing us with Del/Ros1 plants, Dr. Monedero for the gift of scFv and VP8* clones and Dr. Ann Powell and Dr. Anna N. Stepanova for carefully correcting the manuscript. This work was supported by the Spanish Ministry of Science, Technology and Innovation: Grants BIO2008-03434, AGL2009-13388-C03-01 and BIO2010-15384 and P. Juarez FPU fellowship. en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation Spanish Ministry of Science, Technology and Innovation FPU fellowship es_ES
dc.relation Spanish Ministry of Science, Technology and Innovation BIO2008-03434 AGL2009-13388-C03-01 BIO2010-15384 es_ES
dc.relation.ispartof Plant Biotechnology Journal es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Tomato es_ES
dc.subject IgA es_ES
dc.subject Rotavirus es_ES
dc.subject Passive immunization es_ES
dc.subject Identity preservation es_ES
dc.subject Anthocyanins es_ES
dc.subject Plant synthetic biology es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1467-7652.2011.00666.x
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Juárez Ortega, P.; Presa Castro, S.; Espi, J.; Pineda Chaza, BJ.; Antón Martínez, MT.; Moreno Ferrero, V.; Buesa, J.... (2012). Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes. Plant Biotechnology Journal. 10:341-352. doi:10.1111/j.1467-7652.2011.00666.x es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/j.1467-7652.2011.00666.x es_ES
dc.description.upvformatpinicio 341 es_ES
dc.description.upvformatpfin 352 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.relation.senia 233087 es_ES


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