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dc.contributor.author | Martínez Ramos, Cristina | es_ES |
dc.contributor.author | Lebourg, Myriam | es_ES |
dc.date.accessioned | 2016-11-11T11:29:06Z | |
dc.date.available | 2016-11-11T11:29:06Z | |
dc.date.issued | 2015-08 | |
dc.identifier.issn | 1552-4973 | |
dc.identifier.uri | http://hdl.handle.net/10251/73842 | |
dc.description.abstract | Background: Cancer research focuses increasingly on cancer stem cell study as those cells are thought to be the root of chemo and radioresistance of the most aggressive cancer types. Nevertheless, two-dimensional (2D) cell culture and even three-dimensional (3D) spheroid models, with their limited ability to reflect cell-extracellular matrix interactions, are not ideal for the study of cancer stem cells (CSCs). In this study, we establish a 3D in vitro cancer model using a synthetic and natural scaffold with tunable features and show that U87 cells cultured in this system acquire a stem-cell like phenotype. Methods: U87 astrocytoma cells were grown on polycaprolactone (PCL)-2D flat substrates (2D) and PCL-3D scaffolds (3D) eventually containing hyaluronic acid (3D-HA). Cell viability, growth patterns, morphology, and cell surface marker expression (CD44, RHAMM and CD133) were studied to assess the effect of 3D culture and presence of HA. Results: 3D scaffold, but most prominently presence of HA induced changes in cell morphology and marker expression; 3D-HA cultures showed features of aggregates; moreover, markedly increased expression of Nestin, CD44, RHAMM, and CD133 in 3D-HA scaffolds were found. Conclusions: the behavior of U87 in our 3D-HA model is more similar to tumor growth in vivo and a stem-like phenotype is promoted. Thus, the 3D-HA scaffold could provide a useful model for CSCs study and anti-cancer therapeutics research in vitro and may have preclinical application for the screening of drug candidates. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1249-1257, 2015. | es_ES |
dc.description.sponsorship | Contract grant sponsors: National R&D&i Plan (2008-2011; CIBER-BBN), Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, and Instituto de Salud Carlos III (European Regional Development Fund) | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation.ispartof | Journal of Biomedical Materials Research Part B: Applied Biomaterials | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.title | Three-dimensional constructs using hyaluronan cell carrier as a tool for the study of cancer stem cells | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1002/jbm.b.33304 | |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GV%2F2012%2F026/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular | es_ES |
dc.description.bibliographicCitation | Martínez Ramos, C.; Lebourg, M. (2015). Three-dimensional constructs using hyaluronan cell carrier as a tool for the study of cancer stem cells. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 103(6):1249-1257. https://doi.org/10.1002/jbm.b.33304 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1002/jbm.b.33304 | es_ES |
dc.description.upvformatpinicio | 1249 | es_ES |
dc.description.upvformatpfin | 1257 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 103 | es_ES |
dc.description.issue | 6 | es_ES |
dc.relation.senia | 278621 | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |