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Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR

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Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR

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dc.contributor.author Rangoju, P.K.
dc.contributor.author Kumar, S.
dc.contributor.author Kolte, A.P.
dc.contributor.author Gulyani, R.
dc.contributor.author Singh, V.K.
dc.date.accessioned 2010-03-24T12:48:06Z
dc.date.available 2010-03-24T12:48:06Z
dc.date.issued 2007
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/7401
dc.description.abstract [EN] Random amplified polymorphic DNA (RAPD) technique was employed to assess the genetic variation and phylogenetic relationship among three broiler rabbit breeds. Ten individuals from each rabbit breed viz. White Giant (WG), Soviet Chinchilla (SC) and Grey Giant (GG) was taken for the study. Initially, 40 RAPD primers were screened, of which six primers were found polymorphic and they were further utilized to assess the genetic variability among these breeds. The band sharing frequencies (BSF) were computed within and between breeds. The overall BSF value within breed was highest in WG (0.846±0.02) and GG (0.846±0.01), while lowest in SC (0.818±0.02). However, between breeds, BSF value was found higher in SC-GG (0.805± 0.01) followed by WG-SC (0.792±0.02) and WG-GG (0.790±0.02). Overall, there was no significant difference (P>0.05) in BSF values within and between breeds. The BSF value indicated low genetic variability within the breed as compared to between breeds. The Nei's genetic distance (D) was found highest between WG-GG (D=0.1605) followed by WG-SC (D=0.1403) and SC-GG (D=0.1295). The phylogenetic relationship among breeds was analyzed and dendrogram revealed that SC and GG are more closer, while WG-GG are distant to each other. The study suggests that RAPD can be successfully utilized for detecting genetic variation among rabbit breeds. en_EN
dc.language Inglés en_EN
dc.publisher World Rabbit Science. ICTA. UPV en_EN
dc.relation.ispartof World Rabbit Science
dc.rights Reconocimiento - No comercial (by-nc) en_EN
dc.subject Genetic variability en_EN
dc.subject Rapd en_EN
dc.subject Breed en_EN
dc.subject Rabbit en_EN
dc.title Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR en_EN
dc.type Artículo en_EN
dc.identifier.doi 10.4995/wrs.2007.611 es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Rangoju, P.; Kumar, S.; Kolte, A.; Gulyani, R.; Singh, V. (2007). Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR. World Rabbit Science. 15(1):3-8. doi:10.4995/wrs.2007.611 es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2007.611 es_ES
dc.description.upvformatpinicio 3
dc.description.upvformatpfin 8
dc.description.volume 15
dc.description.issue 1
dc.identifier.eissn 1989-8886 es_ES


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