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Design and Assembly Procedures for Large-Sized Biohybrid Scaffolds as Patches for Myocardial Infarct

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Design and Assembly Procedures for Large-Sized Biohybrid Scaffolds as Patches for Myocardial Infarct

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dc.contributor.author Martínez Ramos, Cristina es_ES
dc.contributor.author Rodríguez Pérez, Eduard es_ES
dc.contributor.author Perez Garnes, Manuel es_ES
dc.contributor.author Chachques, Juan Carlos es_ES
dc.contributor.author Moratal Pérez, David es_ES
dc.contributor.author Vallés Lluch, Ana es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.date.accessioned 2016-11-16T09:23:34Z
dc.date.available 2016-11-16T09:23:34Z
dc.date.issued 2014-10-01
dc.identifier.issn 1937-3384
dc.identifier.uri http://hdl.handle.net/10251/74167
dc.description.abstract [EN] Objective: To assemble a biohybrid cardiac patch consisting of a large (5x5 cm) elastomer scaffold whose pores are filled with a self-assembling peptide (SAP) gel entrapping adipose stem cells, to be used as a novel implant in a big animal model (sheep) of myocardial infarction. The study focuses on the way to determine optimal procedures for incorporating the SAP solution and the cells in the patch to ensure cell colonization and a homogeneous cell distribution in the construct before implantation. The problems associated with the scale-up of the different procedures raised by the large size of the construct are discussed. Materials and Methods: Experiments were performed to choose between different assembling alternatives: incorporation of the SAP gel before cell seeding or simultaneous SAP and cell loading of the scaffold; surface seeding of cells or cell injection into the scaffold pores; dissemination of the cells throughout the scaffold before incubation by gentle shaking or by centrifugation. Immunocytochemistry techniques and confocal and scanning electron microscopies were employed to assess and quantify cell colonization of the material and early cell distribution. Cell concentrations and the uniformity of cellular distribution throughout the scaffold were taken as the main criteria to decide between the different alternative procedures. Results: The combination of peptide preloading, cell injection, and shaking before incubation yielded the best results in terms of greater cell density and the most uniform distribution after 24 h of culture compared with the other methods. These techniques could be scaled-up to obtain large biohybrid cardiac patches with success. Conclusions: The results obtained after the different seeding methods allowed us to establish an effective protocol for the assembly of large biohybrid patches for their subsequent implantation in the heart of a big animal model of myocardial infarct in the context of a preclinical study. es_ES
dc.description.sponsorship The authors acknowledge the financing from the European Commission through the ‘‘Regeneration of cardiac tissue assisted by bioactive implants’’ (RECATABI) FP7 NMP3-SL-2009-229239 project. MMP acknowledges support of Instituto de Salud Carlos III with assistance from the European Regional Development Fund through CIBER-BBN initiative.
dc.language Inglés es_ES
dc.publisher Mary Ann Liebert es_ES
dc.relation.ispartof Tissue Engineering Part C Methods es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Design and Assembly Procedures for Large-Sized Biohybrid Scaffolds as Patches for Myocardial Infarct es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1089/ten.TEC.2013.0489
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/229239 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.description.bibliographicCitation Martínez Ramos, C.; Rodríguez Pérez, E.; Perez Garnes, M.; Chachques, JC.; Moratal Pérez, D.; Vallés Lluch, A.; Monleón Pradas, M. (2014). Design and Assembly Procedures for Large-Sized Biohybrid Scaffolds as Patches for Myocardial Infarct. Tissue Engineering Part C Methods. 20(10):817-827. https://doi.org/10.1089/ten.TEC.2013.0489 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1089/ten.tec.2013.0489 es_ES
dc.description.upvformatpinicio 817 es_ES
dc.description.upvformatpfin 827 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 20 es_ES
dc.description.issue 10 es_ES
dc.relation.senia 276580 es_ES
dc.contributor.funder European Commission
dc.contributor.funder Instituto de Salud Carlos III


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