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The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana

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The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana

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dc.contributor.author Contreras-Paredes, Carlos A. es_ES
dc.contributor.author Silva-Rosales, Laura es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.contributor.author Alejandri-Ramirez, Naholi D. es_ES
dc.contributor.author Dinkova, Tzvetanka D. es_ES
dc.date.accessioned 2016-11-25T10:37:20Z
dc.date.available 2016-11-25T10:37:20Z
dc.date.issued 2013-04
dc.identifier.issn 0894-0282
dc.identifier.uri http://hdl.handle.net/10251/74612
dc.description.abstract [EN] Translation initiation factor eIF4E exerts an important role during infection of viral species in the family Potyviridae. Particularly, a eIF(iso)4E family member is required for Arabidopsis thaliana susceptibility to Turnip mosaic virus, Lettuce mosaic virus, and Tobacco etch virus (TEV). In addition, a resistance mechanism named restriction of TEV movement (RTM) in A. thaliana controls the systemic spread of TEV in Col-0 ecotype. Here, we describe that TEV-TAMPS, a Mexican isolate, overcomes the RTM resistance mechanism reported for TEV-7DA infection of the Col-0 ecotype but depends on eIF(iso)4E for its systemic spread. To understand at which level eIF(iso)4E participates in A. thaliana TEV-TAMPS infection, the viral RNA replication and translation were measured. The absence or overexpression of eIF(iso)4E did not affect viral translation, and replication was still observed in the absence of eIF(iso)4E. However, the TEV-TAMPS systemic spread was completely abolished in the null mutant. The viral protein genome-linked (VPg) precursor NIa was found in coimmunoprecipitated complexes with both, eIF(iso)4E and eIF4E. However, the viral coat protein (CP) was only present in the eIF(iso)4E complexes. Since both the VPg and the CP proteins are needed for systemic spread, we propose a role of A. thaliana eIF(iso)4E in the movement of TEV-TAMPS within this host. es_ES
dc.description.sponsorship This work was supported by grants to T. D. Dinkova (PAPIIT IN204309, IN210912 and CONACYT 81708) and L. Silva-Rosales (Cinvestav funds). J.-A. Daros was funded by grant BIO2011-26741 (MEC, Spain). C. A. Contreras-Paredes and N. D. Alejandri-Ramirez were awarded with a fellowship from CONACYT for their Ph.D. studies. The authors are thankful to W. Dougherty for the NIa-TEV antibody and K. Browning (University of Texas at Austin) for Arabidopsis eIF(iso)4E and eIF4E antibodies. en_EN
dc.language Inglés es_ES
dc.publisher American Phytopathological Society es_ES
dc.relation MEC, Spain BIO2011-26741 es_ES
dc.relation CONACYT es_ES
dc.relation PAPIIT IN204309 es_ES
dc.relation IN210912 es_ES
dc.relation CONACYT 81708 es_ES
dc.relation.ispartof Molecular Plant-Microbe Interactions es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject TURNIP-MOSAIC-VIRUS es_ES
dc.subject GENOME-LINKED PROTEIN es_ES
dc.subject LONG-DISTANCE MOVEMENT es_ES
dc.subject RECESSIVE RESISTANCE GENE es_ES
dc.subject IN-VITRO TRANSLATION es_ES
dc.subject COAT PROTEIN es_ES
dc.subject FACTOR EIF4E es_ES
dc.subject POTYVIRUS INFECTION es_ES
dc.subject SUSCEPTIBILITY MUTANTS es_ES
dc.subject TERMINAL REGION es_ES
dc.title The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1094/MPMI-09-12-0225-R
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Contreras-Paredes, CA.; Silva-Rosales, L.; Daros Arnau, JA.; Alejandri-Ramirez, ND.; Dinkova, TD. (2013). The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana. Molecular Plant-Microbe Interactions. 26(4):461-470. doi:10.1094/MPMI-09-12-0225-R es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1094/MPMI-09-12-0225-R es_ES
dc.description.upvformatpinicio 461 es_ES
dc.description.upvformatpfin 470 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 258236 es_ES
dc.identifier.pmid 23252462


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