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Use chemometric techniques in the optimization of a specific bioassay for betalactams in milk

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Use chemometric techniques in the optimization of a specific bioassay for betalactams in milk

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dc.contributor.author Nagel, Orlando Guillermo es_ES
dc.contributor.author Molina Pons, Mª Pilar es_ES
dc.contributor.author Althaus, Rafael Lisandro es_ES
dc.date.accessioned 2016-11-25T11:11:39Z
dc.date.available 2016-11-25T11:11:39Z
dc.date.issued 2012-01
dc.identifier.issn 0266-8254
dc.identifier.uri http://hdl.handle.net/10251/74617
dc.description.abstract Aims: A microbiological bioassay using Geoacillus stearothermophilus was optimized to detect betalactams at concentrations near to the Maximum Residue Limits (MRLs), with low cross-specificity for tetracycline. Methods and Results: A factorial design (3×4) was used to evaluate the effects of concentration of spores (2·0×10 6, 4·0×10 6 and 8·0×10 6 spores ml -1) and incubation time (3·0, 3·5, 4·0 and 4·5h) on the response of the bioassay. Then, desirability function to raise the detection capabilities (CC ß) of tetracyclines and increase sensitivity to betalactams was implemented. Significant effects of Log[S] and incubation time [It] on the CC ß of betalactams and tetracyclines were observed. Finally, high value of global desirability (D=0·853), adequate betalactams CC ß (3·8¿gl -1 of penicillin 'G', 27¿gl -1 of oxacillin, 8·1¿gl -1 of ampicillin, 48¿gl -1 of cloxacillin) and high tetracyclines CC ß (5260¿gl -1 chlortetracycline, 1550¿gl -1 of oxytetracycline, 1070¿gl -1 of tetracycline) were calculated. Conclusions: The application of chemometric tools allows the optimization of a bioassay that detects betalactam residues in milk. The more robust conditions have been achieved in Log[S]=6·30 and [It]=4·20h. Significance and Impact of the Study: The logistic regression model and the desirability function are adequate chemometric techniques to improve the properties of the methods, because it is possible to increase sensitivity and decrease cross-specificity simultaneously. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology. es_ES
dc.description.sponsorship This research work has been carried out as part of the CAD-D'09/12 Proyect (No. 033 - 173 Res. H.C.D.No. 100/09), supported financially by the Universidad Nacional del Litoral (Santa Fe, Republica Argentina). en_EN
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation Universidad Nacional del Litoral (Santa Fe, Republica Argentina) 033 - 173 100/09 es_ES
dc.relation.ispartof Letters in Applied Microbiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Bioassay es_ES
dc.subject Desirability function es_ES
dc.subject Logistic regression model es_ES
dc.subject Milk es_ES
dc.subject Ampicillin es_ES
dc.subject Beta lactam es_ES
dc.subject Chlortetracycline es_ES
dc.subject Cloxacillin es_ES
dc.subject Oxacillin es_ES
dc.subject Oxytetracycline es_ES
dc.subject Penicillin G es_ES
dc.subject Tetracycline es_ES
dc.subject Bacterium es_ES
dc.subject Experimental study es_ES
dc.subject Logistics es_ES
dc.subject Optimization es_ES
dc.subject Regression analysis es_ES
dc.subject Antibiotic sensitivity es_ES
dc.subject Article es_ES
dc.subject Bacterial spore es_ES
dc.subject Chemometrics es_ES
dc.subject Concentration (parameters) es_ES
dc.subject Geoacillus stearothermophilus es_ES
dc.subject Microbiological examination es_ES
dc.subject Nonhuman es_ES
dc.subject Process optimization es_ES
dc.subject Animals es_ES
dc.subject Anti-Bacterial Agents es_ES
dc.subject Beta-Lactams es_ES
dc.subject Biological Assay es_ES
dc.subject Geobacillus stearothermophilus es_ES
dc.subject Logistic Models es_ES
dc.subject Sensitivity and Specificity es_ES
dc.subject Tetracyclines es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Use chemometric techniques in the optimization of a specific bioassay for betalactams in milk es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1472-765X.2011.03169.x
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Nagel, OG.; Molina Pons, MP.; Althaus, RL. (2012). Use chemometric techniques in the optimization of a specific bioassay for betalactams in milk. Letters in Applied Microbiology. 54(1):32-38. https://doi.org/10.1111/j.1472-765X.2011.03169.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/j.1472-765X.2011.03169.x es_ES
dc.description.upvformatpinicio 32 es_ES
dc.description.upvformatpfin 38 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 54 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 209214 es_ES


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