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A combination of direct viable count and fluorescence in situ hibridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

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A combination of direct viable count and fluorescence in situ hibridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

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dc.contributor.author García Hernández, Jorge es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Amorocho Cruz, Claudia Milena es_ES
dc.contributor.author Hernández Pérez, Manuel es_ES
dc.date.accessioned 2016-11-29T07:49:26Z
dc.date.available 2016-11-29T07:49:26Z
dc.date.issued 2011-03
dc.identifier.issn 0266-8254
dc.identifier.uri http://hdl.handle.net/10251/74689
dc.description.abstract [EN] Aims: We have developed a direct viable count (DVC)-FISH procedure for quickly and easily discriminating between viable and nonviable cells of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains, the traditional yogurt bacteria. Methods and Results: direct viable count method has been modified and adapted for Lact. delbrueckii subsp, bulgaricus and Strep. thermophilus analysis by testing different times of incubation and concentrations of DNA-gyrase inhibitors. DVC procedure has been combined with fluorescent in situ hybridization (FISH) for the specific detection of viable cells of both bacteria with specific rRNA oligonucleotide probes (DVC-FISH). Of the four antibiotics tested (novobiocin, nalidixic acid, pipemidic acid and ciprofloxacin), novobiocin was the most effective for DVC method and the optimum incubation time was 7 h for both bacteria. The number of viable cells was obtained by the enumeration of specific hybridized cells that were elongated at least twice their original length for Lactobacillus and twice their original size for Streptococcus. Conclusions: This technique was successfully applied to detect viable cells in inoculated faeces. Significance and Impact of the Study: Results showed that this DVC-FISH procedure is a quick and culture-independent useful method to specifically detect viable Lact, delbrueckii subsp, bulgaricus and Strep thermophilus in different samples, being applied for the first time to lactic acid bacteria. es_ES
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Letters in Applied Microbiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Direct viable count es_ES
dc.subject FISH es_ES
dc.subject Lactobacillus delbrueckii subsp es_ES
dc.subject Bulgaricus es_ES
dc.subject Rapid method es_ES
dc.subject Streptococcus thermophilus es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title A combination of direct viable count and fluorescence in situ hibridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1472-765X.2011.03201.x
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation García Hernández, J.; Moreno Trigos, MY.; Amorocho Cruz, CM.; Hernández Pérez, M. (2011). A combination of direct viable count and fluorescence in situ hibridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Letters in Applied Microbiology. 54(3):247-254. doi:10.1111/j.1472-765X.2011.03201.x es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/j.1472-765X.2011.03201.x es_ES
dc.description.upvformatpinicio 247 es_ES
dc.description.upvformatpfin 254 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 54 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 210888 es_ES


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