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An Immunosensor for the Automatic Determination of the Antifouling Agent Irgarol 1051 in Natural Waters

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An Immunosensor for the Automatic Determination of the Antifouling Agent Irgarol 1051 in Natural Waters

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dc.contributor.advisor We express our gratitude to Sergi Morais for his helpful discussions and the immunoreagents testing, and to Inma Ferrer for carrying out HPLC analysis. This work has been supported by the CICYT (AMB 96-1079), DGCYT (PB 95- 0740), EC (environmental and climate contracts EVN4-CT95-0066 and EVN4-CT97-0476), and RIANA (ENV4-CT95-0066). J. P. acknowledges a grant from the Ministerio de Educacio´n y Ciencia, Spain, to carry out Ph D. studies.
dc.contributor.author González Martínez, Miguel Ángel es_ES
dc.contributor.author Penalva Villegas, José es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author Ballesteros, Berta es_ES
dc.contributor.author Marco, Maria Pilar es_ES
dc.contributor.author Barceló, Damià es_ES
dc.date.accessioned 2016-11-30T11:01:19Z
dc.date.issued 1998-09
dc.identifier.issn 0013-936X
dc.identifier.uri http://hdl.handle.net/10251/74792
dc.description.abstract [EN] The development of an immunosensor for analysis of the antifouling agent Irgarol 1051 (2-methylthio-4-tert-butylamin-6-cyclopropylamin-s-triazine) is described. The immunosensor is based on a heterogeneous competitive enzyme immunoassay and uses binder azlactone support with immobilized protein A/G. The flow-through immunosensor is completely automated and is able to carry out a whole analysis cycle in about 23 min. Competitive calibration curves have an I50 value of 0.053 μg/L (0.21 nM), with a limit of detection of 0.010 μg/L and a quantification dynamic range from 0.019 to 0.158 μg/L. The protein A/G support can be used for more than 600 assay cycles without loss of sensor performance. The sensor is prone to some interferences from tert-butyl-containing s-triazine compounds, such as terbutryn and terbumeton, but little inteference is shown for other s-triazine compounds, e.g., atrazine or simazine. The developed sensor is applied to the determination of trace levels of Irgarol 1051 in water samples such as Mediterranean marina and beach seawater, estuarine river water, and lake water, without any sample pretreatment other than adjusting the pH and ionic strength of samples to those of standards. Good correlation is achieved between the immunosensor results and ELISA or HPLC analyses. es_ES
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation CICYT/AMB 96-1079 es_ES
dc.relation DGICYT/PB 95-0740 es_ES
dc.relation DGICYT/EVN4-CT95-0066 es_ES
dc.relation DGICYT/ENV4-CT95-0066 es_ES
dc.relation DGICYT/EVN4-CT97-0476
dc.relation.ispartof Environmental Science and Technology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Linked-immunosorbent-assay es_ES
dc.subject Coastal waters es_ES
dc.subject Contamination es_ES
dc.subject Immunoassay es_ES
dc.subject Pesticide es_ES
dc.subject Compound es_ES
dc.subject Paints es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title An Immunosensor for the Automatic Determination of the Antifouling Agent Irgarol 1051 in Natural Waters es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/es980120v
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation González Martínez, MÁ.; Penalva Villegas, J.; Puchades, R.; Maquieira Catala, Á.; Ballesteros, B.; Marco, MP.; Barceló, D. (1998). An Immunosensor for the Automatic Determination of the Antifouling Agent Irgarol 1051 in Natural Waters. Environmental Science and Technology. 32(21):3442-3447. doi:10.1021/es980120v es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1021/es980120v es_ES
dc.description.upvformatpinicio 3442 es_ES
dc.description.upvformatpfin 3447 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 32 es_ES
dc.description.issue 21 es_ES
dc.relation.senia 317387 es_ES
dc.contributor.funder Comisión Interministerial de Ciencia y Tecnología (CICYT)
dc.contributor.funder Dirección General de Investigación Científica y Técnica (DGICYT)


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