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Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against Rotavirus

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Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against Rotavirus

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dc.contributor.author Juárez Ortega, Paloma es_ES
dc.contributor.author Fernández Del Carmen, María Asunción es_ES
dc.contributor.author Rambla Nebot, Jose Luis es_ES
dc.contributor.author Presa Castro, Silvia es_ES
dc.contributor.author Mico, Amparo es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2016-12-09T11:32:01Z
dc.date.issued 2014-08-13
dc.identifier.issn 0021-8561
dc.identifier.uri http://hdl.handle.net/10251/75055
dc.description.abstract The production of neutralizing immunoglobulin A (IgA) in edible fruits as a means of oral passive immunization is a promising strategy for the inexpensive treatment of mucosal diseases. This approach is based on the assumption that the edible status remains unaltered in the immunoglobulin-expressing fruit, and therefore extensive purification is not required for mucosal delivery. However, unintended effects associated with IgA expression such as toxic secondary metabolites and protein allergens cannot be dismissed a priori and need to be investigated. This paper describes a collection of independent transgenic tomato lines expressing a neutralizing human IgA against rotavirus, a mucosal pathogen producing severe diarrhea episodes. This collection was used to evaluate possible unintended effects associated with recombinant IgA expression. A comparative analysis of protein and secondary metabolite profiles using wild type lines and other commercial varieties failed to find unsafe features significantly associated with IgA expression. Preliminary, the data indicate that formulations derived from IgA tomatoes are as safe for consumption as equivalent formulations derived from wild type tomatoes. es_ES
dc.description.sponsorship This work has been funded by Grant BIO2010-15384 from Plan Nacional I + D of the Spanish Ministry of Science. P.Juarez is the recipient of a FPU fellowship. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation.ispartof Journal of Agricultural and Food Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tomato es_ES
dc.subject IgA es_ES
dc.subject Antibody es_ES
dc.subject Rotavirus es_ES
dc.subject Unintended effects es_ES
dc.subject Metabolomics es_ES
dc.subject Proteomics es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against Rotavirus es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/jf502292g
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2010-15384/ES/FABRICANDO TOMATES SALUDABLES: BIOPIEZAS PARA INTRAGENESIS Y MOLECULAR FARMING EN SOLANACEAS/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Juárez Ortega, P.; Fernández Del Carmen, MA.; Rambla Nebot, JL.; Presa Castro, S.; Mico, A.; Granell Richart, A.; Orzáez Calatayud, DV. (2014). Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against Rotavirus. Journal of Agricultural and Food Chemistry. 62(32):8158-8168. https://doi.org/10.1021/jf502292g es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1021/jf502292g es_ES
dc.description.upvformatpinicio 8158 es_ES
dc.description.upvformatpfin 8168 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 62 es_ES
dc.description.issue 32 es_ES
dc.relation.senia 284263 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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