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Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity

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Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity

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dc.contributor.author Fernández Del Carmen, María Asunción es_ES
dc.contributor.author Juárez Ortega, Paloma es_ES
dc.contributor.author Presa Castro, Silvia es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2016-12-09T11:48:28Z
dc.date.available 2016-12-09T11:48:28Z
dc.date.issued 2013-02-20
dc.identifier.issn 0168-1656
dc.identifier.uri http://hdl.handle.net/10251/75059
dc.description.abstract The plant kingdom is an underexplored source of valuable proteins which, like plant lectins, display unique interacting specificities. Furthermore, plant protein diversity remains under-exploited due to the low availability and heterogeneity of native sources. All these hurdles could be overcome with recombinant production. A narrow phylogenetic gap between the native source and the recombinant platform is likely to facilitate proper protein processing and stability; therefore, the plant cell chassis should be specially suited for the recombinant production of many plant native proteins. This is illustrated herein with the recombinant production of two representatives of the plant jacalin-related lectin (JRLs) protein family in Nicotiana benthamiana using state-of-the-art magnICON technology. Mannose-specific Banlec JRL was produced at very high levels in leaves, reaching 1.0 mg of purified protein per gram of fresh weight and showing strong agglutination activity. Galactose-specific jacalin JRL, with its complicated processing requirements, was also successfully produced in N. benthamiana at levels of 0.25 mg of purified protein per gram of fresh weight. Recombinant Jacalin (rJacalin) proved efficient in the purification of human IgA1, and was able to discriminate between plant-made and native IgA1 due to their differential glycosylation status. Together, these results show that the plant cell factory should be considered a primary option in the recombinant production of valuable plant proteins. (C) 2012 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship We wish to acknowledge Prof. Gleba and Dr. Klimyuk from ICON Genetics for providing the magnICON expression system. We also thank Prof. R.P. Sharma, University of Hyderabad, India, for kindly providing us with jackfruit seeds and Dr. Luis Rodenas at the UPV Animal Sciences Department for his help with samples for the agglutination analysis. This work has been supported by Grant BIO2010-15384 from MINECO, Spain, and P. Juarez FPU fellowship AP2008-01140. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Journal of Biotechnology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Lectin es_ES
dc.subject Jacalin es_ES
dc.subject Molecular farming es_ES
dc.subject IgA es_ES
dc.subject Viral vectors es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jbiotec.2012.11.017
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2010-15384/ES/FABRICANDO TOMATES SALUDABLES: BIOPIEZAS PARA INTRAGENESIS Y MOLECULAR FARMING EN SOLANACEAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AP2008-01140/ES/AP2008-01140/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Fernández Del Carmen, MA.; Juárez Ortega, P.; Presa Castro, S.; Granell Richart, A.; Orzáez Calatayud, DV. (2013). Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity. Journal of Biotechnology. 163(4):391-400. https://doi.org/10.1016/j.jbiotec.2012.11.017 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jbiotec.2012.11.017 es_ES
dc.description.upvformatpinicio 391 es_ES
dc.description.upvformatpfin 400 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 163 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 257704 es_ES


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