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Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response

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Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response

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dc.contributor.author Rios Garcia, Gabino es_ES
dc.contributor.author Cabedo López, Marc es_ES
dc.contributor.author Rull Costa, Baltasaar es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.contributor.author Mulet Salort, José Miguel es_ES
dc.date.accessioned 2016-12-15T08:40:55Z
dc.date.available 2016-12-15T08:40:55Z
dc.date.issued 2013
dc.identifier.issn 1567-1356
dc.identifier.uri http://hdl.handle.net/10251/75235
dc.description.abstract [EN] We have identified QDR2 in a screening for genes able to confer tolerance to sodium and/or lithium stress upon overexpression. Qdr2 is a multidrug transporter of the major facilitator superfamily, originally described for its ability to transport the antimalarial drug quinidine and the herbicide barban. In order to identify its physiological substrate, we have screened for phenotypes dependent on QDR2 and found that Qdr2 is able to transport monovalent and divalent cations with poor selectivity, as shown by growth tests and the determination of internal cation content. Moreover, strains overexpressing or lacking QDR2 also exhibit phenotypes when reactive oxygen species producing agents, such as hydrogen peroxide or menadione, were added to the growth medium. We have also found that the presence of copper and hydrogen peroxide repress the expression of QDR2. In addition, the copper uptake of a qdr2 mutant strain is similar to a wild type, but the extrusion is clearly impaired. Based on our results, we propose that free divalent copper is the main physiological substrate of Qdr2. As copper is a substrate for several redox reactions that occur within the cytoplasm, this function in copper homeostasis explains its role in the oxidative stress response es_ES
dc.description.sponsorship We are indebted to Prof. A. Maquieira for technical assistance in copper measurements. This work was supported by grants PAID-06-10-1496 of the Universitat Politecnica de Valencia (Valencia, Spain), PROMETEO/2010/038 of the 'Conselleria de Educacion' (Valencia, Spain), by grant BFU2011-30197-C03-03 and by grant BFU2011-22526 from the Ministerio de Ciencia e Innovacion (Madrid, Spain). en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation.ispartof FEMS Yeast Research es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Ion transport es_ES
dc.subject Copper homeostasis es_ES
dc.subject Oxidative stress es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/1567-1364.12013
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-30197-C03-03/ES/PAPEL DEL TRAFICO DE PROTEINAS EN LA HOMEOSTASIS DE IONES Y NUTRIENTES EN LEVADURA Y PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-22526/ES/NUEVOS MECANISMOS DE TRANSMISION DE SEÑALES DURANTE EL METABOLISMO DE GLUCOSA Y LA ACIDIFICACION INTRACELULAR: AMPLIANDO LAS FUNCIONES DE LA PROTEINA FOSFATASA 1 Y LA PROTEINA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F038/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-06-10-1496/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Rios Garcia, G.; Cabedo López, M.; Rull Costa, B.; Yenush, L.; Serrano Salom, R.; Mulet Salort, JM. (2013). Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response. FEMS Yeast Research. 13(1):97-106. https://doi.org/10.1111/1567-1364.12013 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/1567-1364.12013 es_ES
dc.description.upvformatpinicio 97 es_ES
dc.description.upvformatpfin 106 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 232253 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder Universitat Politècnica de València


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