- -

C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis

Show simple item record

Files in this item

dc.contributor.author RODRIGUEZ, LESIA es_ES
dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Díaz, Maira es_ES
dc.contributor.author Rodrigues, Americo es_ES
dc.contributor.author Izquierdo García, Ana Cristina es_ES
dc.contributor.author Peirats-Llobet, Marta es_ES
dc.contributor.author Fernández López, Maria Angeles es_ES
dc.contributor.author Antoni-Alandes, Regina es_ES
dc.contributor.author Fernandez, Daniel es_ES
dc.contributor.author Marquez, J.A es_ES
dc.contributor.author Mulet Salort, José Miguel es_ES
dc.contributor.author Albert, Armando es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.date.accessioned 2016-12-15T09:35:35Z
dc.date.available 2016-12-15T09:35:35Z
dc.date.issued 2014-12
dc.identifier.issn 1040-4651
dc.identifier.uri http://hdl.handle.net/10251/75240
dc.description Supplemental Data: http://www.plantcell.org/content/26/12/4802/suppl/DC1 es_ES
dc.description © 2014 American Society of Plant Biologists es_ES
dc.description.abstract Membrane-delimited abscisic acid (ABA) signal transduction plays a critical role in early ABA signaling, but the molecular mechanisms linking core signaling components to the plasma membrane are unclear. We show that transient calcium-dependent interactions of PYR/PYL ABA receptors with membranes are mediated through a 10-member family of C2-domain ABA-related (CAR) proteins in Arabidopsis thaliana. Specifically, we found that PYL4 interacted in an ABA-independent manner with CAR1 in both the plasma membrane and nucleus of plant cells. CAR1 belongs to a plant-specific gene family encoding CAR1 to CAR10 proteins, and bimolecular fluorescence complementation and coimmunoprecipitation assays showed that PYL4-CAR1 as well as other PYR/PYL-CAR pairs interacted in plant cells. The crystal structure of CAR4 was solved, which revealed that, in addition to a classical calcium-dependent lipid binding C2 domain, a specific CAR signature is likely responsible for the interaction with PYR/PYL receptors and their recruitment to phospholipid vesicles. This interaction is relevant for PYR/PYL function and ABA signaling, since different car triple mutants affected in CAR1, CAR4, CAR5, and CAR9 genes showed reduced sensitivity to ABA in seedling establishment and root growth assays. In summary, we identified PYR/PYL-interacting partners that mediate a transient Ca2+-dependent interaction with phospholipid vesicles, which affects PYR/PYL subcellular localization and positively regulates ABA signaling. es_ES
dc.description.sponsorship We thank Joerg Kudla (University of Munster) for kindly providing plasma membrane markers. This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (Grants BIO2011-23446 to P.L.R and BFU2011-25384 to A. A.; fellowships to L.R., R.A., and A.C.I.-G.; BES-2009- 016569; JAE-DOC contract to M.G.-G.) as well as the Senacyt-Ifarhu (Panama) (fellowship to M.D.). en_EN
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation Ministerio de Ciencia e Innovacion es_ES
dc.relation Fondo Europeo de Desarrollo Regional es_ES
dc.relation Consejo Superior de Investigaciones Cientificas BIO2011-23446 BES-2009-016569 es_ES
dc.relation Senacyt-Ifarhu (Panama) es_ES
dc.relation.ispartof Plant Cell es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject KINASE-C-ALPHA es_ES
dc.subject BIMOLECULAR FLUORESCENCE COMPLEMENTATION es_ES
dc.subject PHOSPHATASES TYPE 2C es_ES
dc.subject ANION CHANNEL SLAC1 es_ES
dc.subject CYTOSOLIC PHOSPHOLIPASE-A2 es_ES
dc.subject DROUGHT RESISTANCE es_ES
dc.subject SIGNALING NETWORK es_ES
dc.subject BINDING DOMAIN es_ES
dc.subject CALCIUM SENSOR es_ES
dc.subject GUARD-CELLS es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1105/tpc.114.129973
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Rodriguez, L.; Gonzalez Guzman, M.; Díaz, M.; Rodrigues, A.; Izquierdo Garcia, AC.; Peirats-Llobet, M.; Fernández López, MA.... (2014). C2-Domain Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic Acid Sensitivity in Arabidopsis. Plant Cell. 26(12):4802-4820. doi:10.1105/tpc.114.129973 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1105/tpc.114.129973 es_ES
dc.description.upvformatpinicio 4802 es_ES
dc.description.upvformatpfin 4820 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26 es_ES
dc.description.issue 12 es_ES
dc.relation.senia 278728 es_ES
dc.identifier.eissn 1532-298X


This item appears in the following Collection(s)

Show simple item record