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dc.contributor.author | Martinez, Fernando | es_ES |
dc.contributor.author | Sardanyes Cayuela, Jose | es_ES |
dc.contributor.author | Elena Fito, Santiago Fco | es_ES |
dc.contributor.author | Daros Arnau, Jose Antonio | es_ES |
dc.date.accessioned | 2016-12-19T09:06:38Z | |
dc.date.available | 2016-12-19T09:06:38Z | |
dc.date.issued | 2011-07 | |
dc.identifier.issn | 0016-6731 | |
dc.identifier.uri | http://hdl.handle.net/10251/75354 | |
dc.description.abstract | [EN] The tremendous evolutionary potential of RNA viruses allows them to thrive despite host defense mechanisms and endows them with properties such as emergence, host switching, and virulence. The frequency of mutant viruses after an infectious process results from the interplay between the error rate of the viral replicase, from purifying mechanisms acting after transcription on aberrant RNAs, and from the amplification dynamics of virus RNA positive (+) and negative (-) strands. Two extreme scenarios describing viral RNA amplification are the geometric growth, in which each RNA strand serves as template for the synthesis of complementary strands with the same efficiency, and the stamping machine, where a strand is reiteratively used as template to synthesize multiple copies of the complementary. The resulting mutation frequencies are completely different, being geometric growth largely more mutagenic than stamping machine. In this work we evaluate the contribution of geometric growth and stamping machine to the overall genome amplification of the plant (+)-strand RNA virus turnip mosaic virus (TuMV). By means of transfection experiments of Nicotiana benthamiana protoplasts with a TuMV cDNA infectious clone and by using strand-specific quantitative real-time PCR, we determined the amplification dynamics of viral (+) and (-) RNA during a single-cell infectious process. A mathematical model describing the amplification of each viral strand was fitted to the data. Analyses of the model parameters showed that TuMV (+) and (-) RNA amplification occurs through a mixed strategy with similar to 93% of genomes produced via stamping machine and only similar to 7% resulting from geometric growth | es_ES |
dc.description.sponsorship | Special thanks are due to Javier Garcia-Andrade for his generous help and useful suggestions in protoplast transfection work, to Javier Carrera for help with the Monte Carlo simulated annealing algorithm, Mark P. Zwart for critical reading of the manuscript, and two anonymous reviewers for insightful comments and suggestions. F. M. is the recipient of a predoctoral fellowship from Universidad Politecnica de Valencia. This work has been supported by grants BIO2008-01986 (J.A.D.) and BFU2009-06993 (S. F. E.) from the Spanish Ministerio de Ciencia e Innovacion, RGP2008/12 from the Human Frontier Science Program Organization and PROMETEO/2010/019 from the Generalitat Valenciana. We also acknowledge support from the Santa Fe Institute. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Genetics Society of America | es_ES |
dc.relation.ispartof | Genetics | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | MUTATION-RATES | es_ES |
dc.subject | STRANDED RNA | es_ES |
dc.subject | VIRAL-RNA | es_ES |
dc.subject | QUANTIFICATION | es_ES |
dc.subject | ROBUSTNESS | es_ES |
dc.subject | MUTANTS | es_ES |
dc.subject | MODE | es_ES |
dc.subject | GENE | es_ES |
dc.subject | PCR | es_ES |
dc.title | Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1534/genetics.111.129114 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2008-01986/ES/INTERACCIONES RNA-PROTEINA EN EL CICLO INFECCIOSO DE PATOGENOS DE RNA DE PLANTAS/ / | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/HFSP//RGP0012%2F2008/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F019/ES/Implicaciones evolutivas de la supresión del silenciamiento del RNA por parte de proteína virales/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BFU2009-06993/ES/Biologia Evolutiva Y De Sistemas De La Emergencia De Fitovirus De Rna/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Martinez, F.; Sardanyes Cayuela, J.; Elena Fito, SF.; Daros Arnau, JA. (2011). Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication. Genetics. 188(3):637-646. https://doi.org/10.1534/genetics.111.129114 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://dx.doi.org/10.1534/genetics.111.129114 | es_ES |
dc.description.upvformatpinicio | 637 | es_ES |
dc.description.upvformatpfin | 646 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 188 | es_ES |
dc.description.issue | 3 | es_ES |
dc.relation.senia | 216044 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Human Frontier Science Program Organization | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |
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