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dc.contributor.author | Mendoza-Poudereux, Isabel![]() |
es_ES |
dc.contributor.author | Muñoz Bertomeu, Jesús![]() |
es_ES |
dc.contributor.author | Arrillaga, Isabel![]() |
es_ES |
dc.contributor.author | Segura, Juan![]() |
es_ES |
dc.date.accessioned | 2017-01-13T13:05:06Z | |
dc.date.available | 2017-01-13T13:05:06Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0176-1617 | |
dc.identifier.uri | http://hdl.handle.net/10251/76767 | |
dc.description.abstract | [EN] Spike lavender (Lavandula latifolia) is an economically important aromatic plant producing essential oils, whose components (mostly monoterpenes) are mainly synthesized through the plastidial methylerythritol 4-phosphate (MEP) pathway. 1-Deoxy-d-xylulose-5-phosphate (DXP) synthase (DXS), that catalyzes the first step of the MEP pathway, plays a crucial role in monoterpene precursors biosynthesis in spike lavender. To date, however, it is not known whether the DXP reductoisomerase (DXR), that catalyzes the conversion of DXP into MEP, is also a rate-limiting enzyme for the biosynthesis of monoterpenes in spike lavender. To investigate it, we generated transgenic spike lavender plants constitutively expressing the Arabidopsis thaliana DXR gene. Although two out of the seven transgenic T0 plants analyzed accumulated more essential oils than the controls, this is hardly imputable to the DXR transgene effect since a clear correlation between transcript accumulation and monoterpene production could not be established. Furthermore, these increased essential oil phenotypes were not maintained in their respective T1 progenies. Similar results were obtained when total chlorophyll and carotenoid content in both T0 transgenic plants and their progenies were analyzed. Our results then demonstrate that DXR enzyme does not play a crucial role in the synthesis of plastidial monoterpene precursors, suggesting that the control flux of the MEP pathway in spike lavender is primarily exerted by the DXS enzyme | es_ES |
dc.description.sponsorship | This work has been funded by the Regional Government of Valencia (PROMETEO/2009/075, Generalitat Valenciana); the Spanish Ministerio de Educacion y Cultura, FPU fellowship to I. M-P (AP2007/04606); and Fundacion Ramon Areces, fellowship to I. M-P. | |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Journal of Plant Physiology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | DXR enzyme | es_ES |
dc.subject | DXS enzyme | es_ES |
dc.subject | Essential oil | es_ES |
dc.subject | Monoterpene | es_ES |
dc.subject | Spike lavender | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Deoxyxylulose 5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in spike lavender | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.jplph.2014.07.012 | |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO09%2F2009%2F075/ES/Mejora de plantas con interés agronómico y forestal- MEPIAF/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//AP2007-04606/ES/AP2007-04606/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Mendoza-Poudereux, I.; Muñoz Bertomeu, J.; Arrillaga, I.; Segura, J. (2014). Deoxyxylulose 5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in spike lavender. Journal of Plant Physiology. 171:1564-1570. https://doi.org/10.1016/j.jplph.2014.07.012 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.jplph.2014.07.012 | es_ES |
dc.description.upvformatpinicio | 1564 | es_ES |
dc.description.upvformatpfin | 1570 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 171 | es_ES |
dc.relation.senia | 270136 | es_ES |
dc.identifier.pmid | 25151124 | |
dc.contributor.funder | Ministerio de Educación y Ciencia | |
dc.contributor.funder | Generalitat Valenciana | |
dc.contributor.funder | Ministerio de Educación | es_ES |