- -

Deoxyxylulose 5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in spike lavender

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Deoxyxylulose 5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in spike lavender

Show simple item record

Files in this item

dc.contributor.author Mendoza-Poudereux, Isabel es_ES
dc.contributor.author Muñoz Bertomeu, Jesús es_ES
dc.contributor.author Arrillaga, Isabel es_ES
dc.contributor.author Segura, Juan es_ES
dc.date.accessioned 2017-01-13T13:05:06Z
dc.date.available 2017-01-13T13:05:06Z
dc.date.issued 2014
dc.identifier.issn 0176-1617
dc.identifier.uri http://hdl.handle.net/10251/76767
dc.description.abstract [EN] Spike lavender (Lavandula latifolia) is an economically important aromatic plant producing essential oils, whose components (mostly monoterpenes) are mainly synthesized through the plastidial methylerythritol 4-phosphate (MEP) pathway. 1-Deoxy-d-xylulose-5-phosphate (DXP) synthase (DXS), that catalyzes the first step of the MEP pathway, plays a crucial role in monoterpene precursors biosynthesis in spike lavender. To date, however, it is not known whether the DXP reductoisomerase (DXR), that catalyzes the conversion of DXP into MEP, is also a rate-limiting enzyme for the biosynthesis of monoterpenes in spike lavender. To investigate it, we generated transgenic spike lavender plants constitutively expressing the Arabidopsis thaliana DXR gene. Although two out of the seven transgenic T0 plants analyzed accumulated more essential oils than the controls, this is hardly imputable to the DXR transgene effect since a clear correlation between transcript accumulation and monoterpene production could not be established. Furthermore, these increased essential oil phenotypes were not maintained in their respective T1 progenies. Similar results were obtained when total chlorophyll and carotenoid content in both T0 transgenic plants and their progenies were analyzed. Our results then demonstrate that DXR enzyme does not play a crucial role in the synthesis of plastidial monoterpene precursors, suggesting that the control flux of the MEP pathway in spike lavender is primarily exerted by the DXS enzyme es_ES
dc.description.sponsorship This work has been funded by the Regional Government of Valencia (PROMETEO/2009/075, Generalitat Valenciana); the Spanish Ministerio de Educacion y Cultura, FPU fellowship to I. M-P (AP2007/04606); and Fundacion Ramon Areces, fellowship to I. M-P.
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation GV/PROMETEO/2009/075 es_ES
dc.relation MEC/FPU/AP2007/04606 es_ES
dc.relation.ispartof Journal of Plant Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject DXR enzyme es_ES
dc.subject DXS enzyme es_ES
dc.subject Essential oil es_ES
dc.subject Monoterpene es_ES
dc.subject Spike lavender es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Deoxyxylulose 5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in spike lavender es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.jplph.2014.07.012
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Mendoza-Poudereux, I.; Muñoz Bertomeu, J.; Arrillaga, I.; Segura, J. (2014). Deoxyxylulose 5-phosphate reductoisomerase is not a rate-determining enzyme for essential oil production in spike lavender. Journal of Plant Physiology. 171:1564-1570. doi:10.1016/j.jplph.2014.07.012 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.jplph.2014.07.012 es_ES
dc.description.upvformatpinicio 1564 es_ES
dc.description.upvformatpfin 1570 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 171 es_ES
dc.relation.senia 270136 es_ES
dc.identifier.pmid 25151124
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Generalitat Valenciana


This item appears in the following Collection(s)

Show simple item record