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dc.contributor.author | Bernardos Bau, Andrea | es_ES |
dc.contributor.author | Mondragón Martínez, Laura | es_ES |
dc.contributor.author | Aznar, Elena | es_ES |
dc.contributor.author | Marcos Martínez, María Dolores | es_ES |
dc.contributor.author | Martínez-Máñez, Ramón | es_ES |
dc.contributor.author | Sancenón Galarza, Félix | es_ES |
dc.contributor.author | Soto Camino, Juan | es_ES |
dc.contributor.author | Barat Baviera, José Manuel | es_ES |
dc.contributor.author | Pérez Payá, Enrique | es_ES |
dc.contributor.author | Guillem, Carmen | es_ES |
dc.contributor.author | Amorós, Pedro | es_ES |
dc.date.accessioned | 2017-01-13T19:50:12Z | |
dc.date.issued | 2010-11 | |
dc.identifier.issn | 1936-0851 | |
dc.identifier.uri | http://hdl.handle.net/10251/76786 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/nn101499d. | es_ES |
dc.description.abstract | The synthesis of new capped silica mesoporous nanoparticles for on-command delivery applications is described. The gate-like functional hybrid systems consisted of nanoscopic MCM-41-based materials functionalized on the pore outlets with different “saccharide” derivatives and a dye contained in the mesopores. A series of hydrolyzed starch products as saccharides were selected. The mesoporous silica nanoparticles S1, S2, and S3 containing the grafted starch derivatives Glucidex 47, Gludicex 39, and Glucidex 29 were synthesized. Additionally, for comparative purposes solid S4 containing lactose was prepared. Delivery studies in pure water in the presence of pancreatin or -D-galactosidase were carried out for S1S3 and S4, respectively. S1, S2, and especially S3 showed very low release in the absence of enzyme, but displayed cargo delivery in the presence of the corresponding enzyme. Moreover, nanoparticles of S1 were used to study the controlled release of the dye in intracellular media. Cell viability assays using HeLa and LLC-PK1 cells indicated that S1 nanoparticles were devoid of unspecific cell toxicity. The endocytosis process for S1 nanoparticle internalization in HeLa cells was confirmed, and the anchored starch was degraded by the lysosomal enzymes. Furthermore, a new mesoporous silica nanoparticle functionalized with Glucidex 47 and loaded with a cytotoxic, S1-DOX, was developed. The cell viability with S1-DOX decreased due to the internalization of the nanoparticle, enzyme-dependent opening of the saccharide molecular gate and the consequent release of the cytotoxic agent. As far as the authors know, this is the first example of enzyme-induced in-cell delivery using capped silica mesoporous nanoparticles. | es_ES |
dc.description.sponsorship | The authors wish to express their gratitude to the Spanish Government (Projects MAT2009 14564 C04 01 MAT2009 14564 C04 04 and BIO2007 60066) and the Generalitat Valencia (Projects PROMETEO/2009/016 and PROMETEO/2010/005) for support A B is grateful to the Universidad Politecnica de Valencia for an FPI grant E A thanks the MICINN for a FPU contract We thank Roquette for the Glucidex samples We would like to thank the CIPF confocal microscopy service for technical support | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | ACS Nano | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Carbohydrates | es_ES |
dc.subject | Enzyme | es_ES |
dc.subject | Intracellular controlled release | es_ES |
dc.subject | Mesoporous | es_ES |
dc.subject | Gate | es_ES |
dc.subject.classification | QUIMICA INORGANICA | es_ES |
dc.subject.classification | TECNOLOGIA DE ALIMENTOS | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.subject.classification | INGENIERIA DE LA CONSTRUCCION | es_ES |
dc.title | Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides" | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1021/nn101499d | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-01/ES/Nanomateriales Hibridos Para El Desarrollo De "Puertas Moleculares" De Aplicacion En Procesos De Reconocimiento Y Terapeutica Y Para La Deteccion De Explosivos/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-04/ES/Nanomateriales Mesoporosos Y Particulados Para El Diseño De Sistemas Sensores, Remediacion Y Aplicaciones Relacionadas Con La Energia/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//BIO2007-60066/ES/BIOLOGIA QUIMICA: APLICACION A INTERACCIONES PROTEINA-PROTEINA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO09%2F2009%2F016/ES/Ayuda prometeo 2009 para el grupo de diseño y desarrollo de sensores/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F005/ES/Identificación de nuevas dianas terapéuticas en angiogénesis y apoptosis basadas en interacciones proteína-proteína/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Gestión en la Edificación - Escola Tècnica Superior de Gestió en l'Edificació | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Grupo de Investigación e Innovación Alimentaria | es_ES |
dc.description.bibliographicCitation | Bernardos Bau, A.; Mondragón Martínez, L.; Aznar, E.; Marcos Martínez, MD.; Martínez-Máñez, R.; Sancenón Galarza, F.; Soto Camino, J.... (2010). Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides". ACS Nano. 4(11):6353-6368. https://doi.org/10.1021/nn101499d | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1021/nn101499d | es_ES |
dc.description.upvformatpinicio | 6353 | es_ES |
dc.description.upvformatpfin | 6368 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 4 | es_ES |
dc.description.issue | 11 | es_ES |
dc.relation.senia | 211858 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |