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Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides"

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Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides"

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dc.contributor.author Bernardos Bau, Andrea es_ES
dc.contributor.author Mondragón Martínez, Laura es_ES
dc.contributor.author Aznar, Elena es_ES
dc.contributor.author Marcos Martínez, María Dolores es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.contributor.author Soto Camino, Juan es_ES
dc.contributor.author Barat Baviera, José Manuel es_ES
dc.contributor.author Pérez Payá, Enrique es_ES
dc.contributor.author Guillem, Carmen es_ES
dc.contributor.author Amorós, Pedro es_ES
dc.date.accessioned 2017-01-13T19:50:12Z
dc.date.issued 2010-11
dc.identifier.issn 1936-0851
dc.identifier.uri http://hdl.handle.net/10251/76786
dc.description This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/nn101499d. es_ES
dc.description.abstract The synthesis of new capped silica mesoporous nanoparticles for on-command delivery applications is described. The gate-like functional hybrid systems consisted of nanoscopic MCM-41-based materials functionalized on the pore outlets with different “saccharide” derivatives and a dye contained in the mesopores. A series of hydrolyzed starch products as saccharides were selected. The mesoporous silica nanoparticles S1, S2, and S3 containing the grafted starch derivatives Glucidex 47, Gludicex 39, and Glucidex 29 were synthesized. Additionally, for comparative purposes solid S4 containing lactose was prepared. Delivery studies in pure water in the presence of pancreatin or -D-galactosidase were carried out for S1S3 and S4, respectively. S1, S2, and especially S3 showed very low release in the absence of enzyme, but displayed cargo delivery in the presence of the corresponding enzyme. Moreover, nanoparticles of S1 were used to study the controlled release of the dye in intracellular media. Cell viability assays using HeLa and LLC-PK1 cells indicated that S1 nanoparticles were devoid of unspecific cell toxicity. The endocytosis process for S1 nanoparticle internalization in HeLa cells was confirmed, and the anchored starch was degraded by the lysosomal enzymes. Furthermore, a new mesoporous silica nanoparticle functionalized with Glucidex 47 and loaded with a cytotoxic, S1-DOX, was developed. The cell viability with S1-DOX decreased due to the internalization of the nanoparticle, enzyme-dependent opening of the saccharide molecular gate and the consequent release of the cytotoxic agent. As far as the authors know, this is the first example of enzyme-induced in-cell delivery using capped silica mesoporous nanoparticles. es_ES
dc.description.sponsorship The authors wish to express their gratitude to the Spanish Government (Projects MAT2009 14564 C04 01 MAT2009 14564 C04 04 and BIO2007 60066) and the Generalitat Valencia (Projects PROMETEO/2009/016 and PROMETEO/2010/005) for support A B is grateful to the Universidad Politecnica de Valencia for an FPI grant E A thanks the MICINN for a FPU contract We thank Roquette for the Glucidex samples We would like to thank the CIPF confocal microscopy service for technical support en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation.ispartof ACS Nano es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Carbohydrates es_ES
dc.subject Enzyme es_ES
dc.subject Intracellular controlled release es_ES
dc.subject Mesoporous es_ES
dc.subject Gate es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification TECNOLOGIA DE ALIMENTOS es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.subject.classification INGENIERIA DE LA CONSTRUCCION es_ES
dc.title Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides" es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1021/nn101499d
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-01/ES/Nanomateriales Hibridos Para El Desarrollo De "Puertas Moleculares" De Aplicacion En Procesos De Reconocimiento Y Terapeutica Y Para La Deteccion De Explosivos/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MAT2009-14564-C04-04/ES/Nanomateriales Mesoporosos Y Particulados Para El Diseño De Sistemas Sensores, Remediacion Y Aplicaciones Relacionadas Con La Energia/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BIO2007-60066/ES/BIOLOGIA QUIMICA: APLICACION A INTERACCIONES PROTEINA-PROTEINA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO09%2F2009%2F016/ES/Ayuda prometeo 2009 para el grupo de diseño y desarrollo de sensores/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F005/ES/Identificación de nuevas dianas terapéuticas en angiogénesis y apoptosis basadas en interacciones proteína-proteína/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Gestión en la Edificación - Escola Tècnica Superior de Gestió en l'Edificació es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Grupo de Investigación e Innovación Alimentaria es_ES
dc.description.bibliographicCitation Bernardos Bau, A.; Mondragón Martínez, L.; Aznar, E.; Marcos Martínez, MD.; Martínez-Máñez, R.; Sancenón Galarza, F.; Soto Camino, J.... (2010). Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides". ACS Nano. 4(11):6353-6368. https://doi.org/10.1021/nn101499d es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1021/nn101499d es_ES
dc.description.upvformatpinicio 6353 es_ES
dc.description.upvformatpfin 6368 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 4 es_ES
dc.description.issue 11 es_ES
dc.relation.senia 211858 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Universitat Politècnica de València es_ES


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