- -

The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes

Show simple item record

Files in this item

dc.contributor.author Peiró Morell, Ana es_ES
dc.contributor.author Martínez-Gil, Luís es_ES
dc.contributor.author Tamborero, Silvia es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Sanchez Navarro, Jesus Angel es_ES
dc.contributor.author Mingarro, Ismael es_ES
dc.date.accessioned 2017-01-23T11:54:43Z
dc.date.available 2017-01-23T11:54:43Z
dc.date.issued 2014-05
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/77215
dc.description.abstract Plant positive-strand RNA viruses require association with plant cell endomembranes for viral translation and replication, as well as for intra- and intercellular movement of the viral progeny. The membrane association and RNA binding of the Tobacco mosaic virus (TMV) movement protein (MP) are vital for orchestrating the macromolecular network required for virus movement. A previously proposed topological model suggests that TMV MP is an integral membrane protein with two putative -helical transmembrane (TM) segments. Here we tested this model using an experimental system that measured the efficiency with which natural polypeptide segments were inserted into the ER membrane under conditions approximating the in vivo situation, as well as in planta. Our results demonstrated that the two hydrophobic regions (HRs) of TMV MP do not span biological membranes. We further found that mutations to alter the hydrophobicity of the first HR modified membrane association and precluded virus movement. We propose a topological model in which the TMV MP HRs intimately associate with the cellular membranes, allowing maximum exposure of the hydrophilic domains of the MP to the cytoplasmic cellular components. es_ES
dc.description.sponsorship This work was supported by grants BFU2009-08401 and BFU2012-39482 (to I. M.) and BIO2011-25018 (to V. P.) from the Spanish MINECO. A. P. is the recipient of a JAE predoc position (CSIC). en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation MINECO BFU2009-08401 es_ES
dc.relation MINECO BFU2012- 39482 es_ES
dc.relation MINECO BIO2011-25018 es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.title The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.03648-13
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Peiró Morell, A.; Martínez-Gil, L.; Tamborero, S.; Pallás Benet, V.; Sanchez Navarro, JA.; Mingarro, I. (2014). The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes. Journal of Virology. 88(5):3016-3026. doi:10.1128/JVI.03648-13 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/JVI.03648-13 es_ES
dc.description.upvformatpinicio 3016 es_ES
dc.description.upvformatpfin 3026 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 88 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 285488 es_ES
dc.identifier.pmid 24371064 en_EN
dc.identifier.pmcid PMC3958068 en_EN


This item appears in the following Collection(s)

Show simple item record