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Expression of B-type natriuretic peptide forms in ischemic human hearts

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Expression of B-type natriuretic peptide forms in ischemic human hearts

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dc.contributor.author Cortes, Raquel es_ES
dc.contributor.author Rosello-Lleti, Esther es_ES
dc.contributor.author Rivera, Miguel es_ES
dc.contributor.author Martinez-Dolz, Luis es_ES
dc.contributor.author Salvador, Antonio es_ES
dc.contributor.author Sirera Pérez, Rafael es_ES
dc.contributor.author Portoles, Manuel es_ES
dc.date.accessioned 2017-01-24T12:58:48Z
dc.date.available 2017-01-24T12:58:48Z
dc.date.issued 2012-07-12
dc.identifier.issn 0167-5273
dc.identifier.uri http://hdl.handle.net/10251/77270
dc.description.abstract Background: This study sought to determine pro-B-type natriuretic peptide (proBNP), BNP, N-terminal proBNP (NT-proBNP) and GATA-4 in the same cardiac tissue, the correlation among them, and the influence of ischemic etiology on their levels. Methods: Protein levels were analyzed by Western blot techniques and mRNA expression was quantified by quantitative real-time polymerase chain reaction (RT-PCR) in a total of 33 human samples from ischemic (ICM), and control hearts. Results: Tissue protein level of proBNP is 1.5- and 12-fold higher than BNP or NT-proBNP respectively (p < 0.0001), and BNP protein level was 8-fold higher than that of NT-proBNP (p < 0.0001) in ICM hearts. Furthermore, proBNP mRNA expression was also increased in ICM (4-fold) compared to control hearts (p < 0.05), but there was not a significant increase in GATA-4 mRNA. Then, tissue NP forms showed a high correlation among them (proBNP vs. BNP r = 0.74, p < 0.0001; proBNP vs. NT-proBNP r = 0.43, p = 0.03; and BNP vs. NT-proBNP r = 0.61, p = 0.001, respectively). Furthermore, GATA-4 with proBNP (r = 0.536, p = 0.007) and BNP (r = 0.610, p = 0.001) in ischemic samples. Finally, we found that proBNP, BNP, NT-proBNP and GATA-4 were increased in our ICM hearts (by 14%, p = 0.004; 46%, p = 0.024, 33%, p = 0.002, and 49%, p = 0.026, respectively) compared with controls. Conclusions: This study shows higher protein level of proBNP in human hearts than of BNP and NT-proBNP, increased proBNP mRNA expression in ICM samples, and a good correlation among tissue natriuretic peptide and GATA-4. Finally, ICM shows a high tissue protein level of proBNP, BNP, NT-proBNP and GATA-4. © 2011 Elsevier Ireland Ltd. All rights reserved. es_ES
dc.description.sponsorship The work was supported by the National Institute of Health Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III, [REDIN-SCOR 06/0003/1001, Project PI07/0462]; and Escuela Valenciana de Estudios para la Salud, [EVES AP039/2007], Spain. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Escuela Valenciana de Estudios para la Salud, Spain EVES AP039/2007 es_ES
dc.relation National Institute of Health Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III [REDIN-SCOR 06/0003/1001, Project PI07/0462] es_ES
dc.relation.ispartof International Journal of Cardiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Expression es_ES
dc.subject Heart failure es_ES
dc.subject Natriuretic peptides es_ES
dc.subject Tissue es_ES
dc.subject Amino terminal pro brain natriuretic peptide es_ES
dc.subject Brain natriuretic peptide es_ES
dc.subject Messenger RNA es_ES
dc.subject Transcription factor GATA 4 es_ES
dc.subject Adult es_ES
dc.subject Article es_ES
dc.subject Clinical article es_ES
dc.subject Controlled study es_ES
dc.subject Disease classification es_ES
dc.subject Female es_ES
dc.subject Heart es_ES
dc.subject Human es_ES
dc.subject Ischemic cardiomyopathy es_ES
dc.subject Ischemic heart disease es_ES
dc.subject Male es_ES
dc.subject Priority journal es_ES
dc.subject Protein analysis es_ES
dc.subject Protein determination es_ES
dc.subject Protein expression es_ES
dc.subject Protein folding es_ES
dc.subject Real time polymerase chain reaction es_ES
dc.subject Western blotting es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Expression of B-type natriuretic peptide forms in ischemic human hearts es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.ijcard.2011.01.014
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Cortes, R.; Rosello-Lleti, E.; Rivera, M.; Martinez-Dolz, L.; Salvador, A.; Sirera Pérez, R.; Portoles, M. (2012). Expression of B-type natriuretic peptide forms in ischemic human hearts. International Journal of Cardiology. 158(2):199-204. doi:10.1016/j.ijcard.2011.01.014 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.ijcard.2011.01.014 es_ES
dc.description.upvformatpinicio 199 es_ES
dc.description.upvformatpfin 204 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 158 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 231613 es_ES


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