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Does storage time in LN2 influence survival and pregnancy outcome of vitrified rabbit embryos?

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Does storage time in LN2 influence survival and pregnancy outcome of vitrified rabbit embryos?

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dc.contributor.author Lavara García, Raquel es_ES
dc.contributor.author Baselga Izquierdo, Manuel es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2017-02-21T12:40:11Z
dc.date.available 2017-02-21T12:40:11Z
dc.date.issued 2011-09
dc.identifier.issn 0093-691X
dc.identifier.uri http://hdl.handle.net/10251/78115
dc.description.abstract Vitrification is one of the most widely used techniques for embryo cryopreservation. The aim of this work was to study the effect of storage time in liquid nitrogen on vitrified rabbit embryos. A total of 1467 vitrified rabbit embryos were transferred into 174 females. The embryos had been maintained in liquid nitrogen during 3 different periods, A) 15 years (32 transfers, 280 embryos). A generalized linear model was used to determine the effect of period on pregnancy and birth rates. A Bayesian approach was applied to analyze the survival rate of the vitrified embryos. In all analyses the number of transferred embryos was included as covariate. It was observed that neither the period of storage nor the number of transferred embryos affected pregnancy rate, and all periods presented similar pregnancy rates (0.85 0.04; 0.86 0.05; 0.78 0.07 for A, B and C). Fertility at birth was affected by the number of transferred embryos, but non-significant differences between periods were detected (0.77 0.04, 0.75 0.07; 0.69 0.08 for A, B and C). Also, the posterior means (highest posterior density intervals at 95%) of embryo survival at birth from pregnant females were similar between the different periods (47 [41 53]; 47 [38 56]; 42 [31 54]; for A, B and C). Results obtained in the present experiment point out that vitrified embryos could be stored in liquid nitrogen during at least fifteen years, achieving good pregnancy rate, fertility and survival at birth. © 2011 Elsevier Inc. es_ES
dc.description.sponsorship This study was supported by the Generalitat Valenciana research program (Prometeo 2009/125) and Spanish Research Project (CICYT AGL2008-03274). Raquel Lavara was supported by a research grant from Spanish Ministry of Education (MEC, FPU AP2007-03755). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Generalitat Valenciana [Prometeo 2009/125] es_ES
dc.relation Spanish Research Project (CICYT) [AGL2008-03274] es_ES
dc.relation Spanish Ministry of Education (MEC) [AP2007-03755] es_ES
dc.relation.ispartof Theriogenology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Embryo transfer es_ES
dc.subject Rabbit es_ES
dc.subject Storage es_ES
dc.subject Vitrification es_ES
dc.subject Animal es_ES
dc.subject Animal disease es_ES
dc.subject Article es_ES
dc.subject Bayes theorem es_ES
dc.subject Cryopreservation es_ES
dc.subject Embryo development es_ES
dc.subject Female es_ES
dc.subject Methodology es_ES
dc.subject Newborn es_ES
dc.subject Physiology es_ES
dc.subject Pregnancy es_ES
dc.subject Prenatal development es_ES
dc.subject Statistical model es_ES
dc.subject Animals es_ES
dc.subject Animals, Newborn es_ES
dc.subject Embryonic Development es_ES
dc.subject Linear Models es_ES
dc.subject Rabbits es_ES
dc.subject Oryctolagus cuniculus es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Does storage time in LN2 influence survival and pregnancy outcome of vitrified rabbit embryos? es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.theriogenology.2011.03.018
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.description.bibliographicCitation Lavara García, R.; Baselga Izquierdo, M.; Vicente Antón, JS. (2011). Does storage time in LN2 influence survival and pregnancy outcome of vitrified rabbit embryos?. Theriogenology. 76(4):652-657. doi:10.1016/j.theriogenology.2011.03.018 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.theriogenology.2011.03.018 es_ES
dc.description.upvformatpinicio 652 es_ES
dc.description.upvformatpfin 657 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 76 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 193955 es_ES


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