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Irreversible electroporation of the liver: is there a safe limit to the ablation volume?

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Irreversible electroporation of the liver: is there a safe limit to the ablation volume?

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dc.contributor.author Sánchez-Velázquez, Patricia es_ES
dc.contributor.author Castellví, Quim es_ES
dc.contributor.author Villanueva, A es_ES
dc.contributor.author Quesada, Rita es_ES
dc.contributor.author Pañella, C. es_ES
dc.contributor.author Cáceres, Marta es_ES
dc.contributor.author Dorcaratto, Dimitri es_ES
dc.contributor.author Andaluz, Anna es_ES
dc.contributor.author Moll, X. es_ES
dc.contributor.author Trujillo Guillen, Macarena es_ES
dc.contributor.author Burdío Pinilla, José Miguel es_ES
dc.contributor.author Berjano Zanón, Enrique es_ES
dc.contributor.author Grande, Luis es_ES
dc.contributor.author Ivorra, A. es_ES
dc.contributor.author Burdío Pinilla, Fernando es_ES
dc.date.accessioned 2017-02-28T18:37:06Z
dc.date.available 2017-02-28T18:37:06Z
dc.date.issued 2016-04-01
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10251/78377
dc.description.abstract Irreversible electroporation is a fast-growing liver ablation technique. Although safety has been well documented in small ablations, our aim is to assess its safety and feasibility when a large portion of liver is ablated. Eighty-seven mice were subjected to high voltage pulses directly delivered across parallel plate electrodes comprising around 40% of mouse liver. One group consisted in 55 athymicnude, in which a tumor from the KM12C cell line was grown and the other thirty-two C57-Bl6 nontumoral mice. Both groups were subsequently divided into subsets according to the delivered field strength (1000V/cm, 2000V/cm) and whether or not they received anti-hyperkalemia therapy. Early mortality (less than 24hours post-IRE) in the 2000V/cm group was observed and revealed considerably higher mean potassium levels. In contrast, the animals subjected to a 2000V/cm field treated with the anti-hyperkalemia therapy had higher survival rates (OR=0.1, 95%CI=0.02–0.32, p<0.001). Early mortality also depended on the electric field magnitude of the IRE protocol, as mice given 1000V/cm survived longer than those given 2000V/cm (OR=4.7, 95%CI=1.8–11.8, p=0.001). Our findings suggest that ionic disturbances, mainly due to potassium alterations, should be warned and envisioned when large volume ablations are performed by IRE. es_ES
dc.description.sponsorship This research was supported by the Spanish "Plan Estatal de Investigacion, Desarrollo e Innovacion Orientada a los Retos de la Sociedad" under Grant TEC2014-52383-C3 (TEC2014-52383-C3-1-R, TEC2014-52383-C3-2-R, and TEC2014-52383-C3-3-R), and by the Spanish "Plan Nacional de I + D + i del Ministerio de Ciencia e Innovacion" under Grants TEC2011-27133-C02 and TEC2010-17285. en_EN
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation “Plan Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad” under Grant TEC2014–52383-C3 (TEC2014-52383-C3-1-R, TEC2014-52383-C3- 2-R, and TEC2014-52383-C3-3-R) es_ES
dc.relation “Plan Nacional de I+D+i del Ministerio de Ciencia e Innovación” under Grants TEC2011-27133-C02 and TEC2010-17285 es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject.classification INGENIERIA MECANICA es_ES
dc.subject.classification MATEMATICA APLICADA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Irreversible electroporation of the liver: is there a safe limit to the ablation volume? es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/srep23781
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Arquitectura - Escola Tècnica Superior d'Arquitectura es_ES
dc.description.bibliographicCitation Sánchez-Velázquez, P.; Castellví, Q.; Villanueva, A.; Quesada, R.; Pañella, C.; Cáceres, M.; Dorcaratto, D.... (2016). Irreversible electroporation of the liver: is there a safe limit to the ablation volume?. Scientific Reports. 6(23781):1-7. doi:10.1038/srep23781 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/srep23781 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 7 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 23781 es_ES
dc.relation.senia 305512 es_ES
dc.identifier.pmid 27032535 en_EN
dc.identifier.pmcid PMC4817133 en_EN
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