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Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast

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Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast

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dc.contributor.author Rienzo, Alessandro es_ES
dc.contributor.author Poveda Huertes, Daniel es_ES
dc.contributor.author Aydin, Selcan es_ES
dc.contributor.author Buchler, Nicolas E. es_ES
dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.date.accessioned 2017-03-13T10:57:18Z
dc.date.available 2017-03-13T10:57:18Z
dc.date.issued 2015-11
dc.identifier.issn 0270-7306
dc.identifier.uri http://hdl.handle.net/10251/78703
dc.description.abstract Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galactose concentrations, correlating with a dynamic change of histone remodeling and RNA polymerase II (RNAPII) association. This behavior is the result of a heterogeneous induction delay caused by decreasing inducer concentrations across the population. Chromatin remodeling appears to be the basis for the dynamic GAL1 expression, because mutants with impaired histone dynamics show severely truncated dose-response profiles. In contrast, the GRE2 promoter operates like a rapid off/on switch in response to increasing osmotic stress, with almost constant expression rates and exclusively temporal regulation of histone remodeling and RNAPII occupancy. The Gal3 inducer and the Hog1 mitogen-activated protein (MAP) kinase seem to determine the different dose-response strategies at the two promoters. Accordingly, GAL1 becomes highly sensitive and dose independent if previously stimulated because of residual Gal3 levels, whereas GRE2 expression diminishes upon repeated stimulation due to acquired stress resistance. Our analysis reveals important differences in the way dynamic signals create dose-sensitive gene expression outputs. es_ES
dc.description.sponsorship This work was supported by grants from Ministerio de Economia y Competitividad (BFU2011-23326), Generalitat de Valencia (ACOMP2011/031), and the NIH Director's New Innovator Award (DP2 OD008654-01). Alessandro Rienzo was a recipient of a predoctoral FPI grant from Ministerio de Economia y Competitividad. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Molecular and Cellular Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject RNA-polymerase-II es_ES
dc.subject Graded transcriptional responses es_ES
dc.subject Saccharomyces cerevisiae es_ES
dc.subject In-vivo es_ES
dc.subject MAP kinase es_ES
dc.subject Expression program es_ES
dc.subject Nuclear periphery es_ES
dc.subject Oxidative stress es_ES
dc.subject Cellular memory es_ES
dc.subject Osmotic stress es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/MCB.00729-15
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-23326/ES/REGULACION DE LA CROMATINA Y DE LA ESTRUCTURA MITOCONDRIAL EN RESPUESTA A ESTRES OSMOTICO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//DP2 OD008654-01/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACOMP%2F2011%2F031/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Rienzo, A.; Poveda Huertes, D.; Aydin, S.; Buchler, NE.; Pascual-Ahuir Giner, MD.; Proft, MH. (2015). Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast. Molecular and Cellular Biology. 35(21):3669-3683. https://doi.org/10.1128/MCB.00729-15 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/MCB.00729-15 es_ES
dc.description.upvformatpinicio 3669 es_ES
dc.description.upvformatpfin 3683 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 35 es_ES
dc.description.issue 21 es_ES
dc.relation.senia 303448 es_ES
dc.identifier.eissn 1098-5549
dc.identifier.pmcid PMC4589597 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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